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A maldi-tof-ms-based identification method and system for slaughtered meat

A technology for live slaughter and identification of results, applied in character and pattern recognition, instruments, analytical materials, etc., can solve the problems of low accuracy and poor reproducibility of detection methods, achieve high stability, good reproducibility, reduce self- The effect of factor interference

Active Publication Date: 2019-12-20
融智生物科技(青岛)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, in order to solve the problems of poor reproducibility and low accuracy of existing detection methods and establish a scientific, efficient and accurate identification method and system, it is necessary to provide a method and system for identifying dead meat based on MALDI-TOF-MS

Method used

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  • A maldi-tof-ms-based identification method and system for slaughtered meat
  • A maldi-tof-ms-based identification method and system for slaughtered meat
  • A maldi-tof-ms-based identification method and system for slaughtered meat

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0117] (1) Prepare meat infusion.

[0118] 20 batches of live slaughtered pork samples were obtained from a designated slaughtering and processing enterprise, and 6 samples in each batch were tested in parallel, totaling 120 samples. After removing fat and connective tissue from each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 25g into a sterile homogeneous bag with a strainer Add 250mL of double distilled water, beat on a beating homogenizer for 2 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0119] (2) MALDI-TOF measurement.

[0120] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sampled, 2 μL for each target. After drying the crystals, put them into a MALDI-TOF instrument, scan and measure, ...

example 2

[0125] (1) Prepare meat infusion.

[0126] 15 batches of diseased and dead pork were obtained from 2 pig farms, and 6 parallel tests were performed on each batch of samples, and the number of samples was 90. After removing fat and connective tissue from each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 10g into a sterile homogeneous bag with a filter Add 100mL of double-distilled water, beat on a beating homogenizer for 3 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0127] (2) MALDI-TOF measurement.

[0128] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sampled, 1 μL for each target. After drying the crystals, put them into a MALDI-TOF instrument, scan and measure, and obtain a mass spectrum. ...

example 3

[0133] (1) Prepare meat infusion.

[0134] A total of 30 batches of pork samples were obtained, of which 15 batches were diseased and dead pork obtained from 4 pig farms, and 15 batches were live slaughtered pigs obtained from a designated slaughterhouse in Hebei Province. Each batch of samples was tested in parallel 6, the number of samples is 180. After removing fat and connective tissue of each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 20g into a sterile homogeneous bag with filter Add 200mL of double-distilled water, beat on a beating homogenizer for 2 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0135] (2) MALDI-TOF measurement.

[0136] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sa...

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Abstract

The invention discloses a MALDI-TOF-MS-based identification method for slaughtered meat, comprising: preparing a sample set, the sample set including a training sample set, a test sample set and a sample set to be tested; applying MALDI-TOF-MS to the sample set Measure to obtain the corresponding mass spectrum; establish a neural network classifier based on the mass spectrum of the training sample set and the test sample set; use the neural network classifier to classify the samples in the test sample set to obtain the identification result. The invention also discloses a MALDI-TOF-MS-based identification system for slaughtered meat. The present invention finds the metabolomics and proteomics differences between dead slaughtered meat and live slaughtered meat at the molecular level by means of a mass spectrometer, and minimizes the interference of environmental factors and sample factors through the training, learning and inspection of neural network classifiers. The detection process has high stability, sensitivity and accuracy, and the detection result has good reproducibility. It is a scientific, accurate and efficient dead meat identification method and system.

Description

technical field [0001] The invention relates to a meat quality identification method and system. More specifically, it relates to a method and system for identifying dead meat based on MALDI-TOF-MS. Background technique [0002] With the improvement of modern living standards, people's demand for high-quality meat is becoming stronger and stronger. Compared with the live slaughtered meat that is purchased and slaughtered live animals and enters the market after quarantine, dead slaughtered meat is a kind of meat that privately purchases and slaughters sick and dead animals and escapes quarantine and enters the market. It has biological hazards, toxic and harmful substances, and The hazards of drug residues pose a great threat to people's quality of life. Therefore, more and more attention has been paid to the inspection and identification of dead slaughtered meat in the market. [0003] Taking dead pork as an example, there are mainly two methods for testing dead pork on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N27/64G06K9/62
CPCG01N27/64G01N33/6851G06F18/214G06F18/241
Inventor 佟雪梅宋合兴刘元杰李运涛周晓光
Owner 融智生物科技(青岛)有限公司
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