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Vaccine composition and preparation method thereof

A vaccine composition and substance technology, applied in biochemical equipment and methods, vaccines, multivalent vaccines, etc., can solve the problems of high production cost and low virus titer, and achieve the effect of good immune effect

Active Publication Date: 2017-11-07
广东渔跃生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, avian adenovirus type 4 and avian reovirus type 2 are mainly propagated through SPF chicken embryo vectors, the virus titer is not high, and the production cost of large-scale production with SPF chicken embryo vectors is very expensive; Passage cell-derived cells to propagate the virus, and then use it to produce inactivated vaccines

Method used

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  • Vaccine composition and preparation method thereof
  • Vaccine composition and preparation method thereof
  • Vaccine composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 A kind of vaccine composition

[0033] The vaccine composition is composed of avian influenza virus H9 subtype antigen, avian adenovirus type 4 antigen, avian reovirus type 2 antigen, avian leukemia J type antigen and adjuvant.

Embodiment 2

[0034] Embodiment 2 A kind of preparation method of vaccine composition

[0035] The preparation method of described vaccine composition comprises the following steps:

[0036] S1. Cell preparation: Digest and disperse LMH cells with trypsin, and use DMEM medium containing 10% v / v newborn bovine serum and 1.0% v / v double antibody at 37°C, 5% CO 2 After culturing to a monolayer of cells, the cells were washed three times with serum-free DMEM medium for inoculation of avian influenza virus H9 subtype, avian adenovirus type 4 and avian reovirus type 2 virus; DF-1 cells were inoculated with pancreatic Digest and disperse with enzymes, use DMEM / F12 culture medium containing 10% v / v newborn bovine serum and 1.0% v / v double antibody at 37°C, 5% CO 2 After culturing to a single layer of cells, wash the cells with serum-free DMEM medium for 3 times for inoculation of avian leukosis virus type J;

[0037] S2. Virus inoculation and cultivation: Inoculate the LMH cells prepared in step ...

Embodiment 3

[0044] Embodiment 3 A kind of preparation method of vaccine composition

[0045] The preparation method of described vaccine composition comprises the following steps:

[0046] S1. Cell preparation: Digest and disperse LMH cells with trypsin, and use DMEM medium containing 5% v / v newborn bovine serum and 1.0% v / v double antibody at 37°C, 5% CO 2 After culturing to a monolayer of cells, the cells were washed three times with serum-free DMEM medium for inoculation of avian influenza virus H9 subtype, avian adenovirus type 4 and avian reovirus type 2 virus; DF-1 cells were inoculated with pancreatic Digest and disperse with enzyme, use DMEM / F12 culture medium containing 10% v / v newborn bovine serum and 0.8% v / v double antibody at 37°C, 5% CO 2 After culturing to a single layer of cells, wash the cells with serum-free DMEM medium for 3 times for inoculation of avian leukosis virus type J;

[0047] S2. Virus inoculation and cultivation: Inoculate the LMH cells prepared in step S1...

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PUM

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Abstract

The invention belongs to the technical field of biological products for animals and particularly relates to a vaccine composition and a preparation method thereof. The vaccine composition comprises avian influenza virus H9 subtype antigens, aviadenovirus 4-type antigens, avian reovirus 2-type virus antigens, avian leukosis J-type antigens and an adjuvant. The vaccine composition has good immune effect for the avian influenza virus H9 subtype antigens, the aviadenovirus 4-type antigens, the avian reovirus 2-type virus antigens and the avian leukosis J-type antigens.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a vaccine composition and a preparation method thereof. Background technique [0002] H9N2 subtype avian influenza belongs to low pathogenicity avian influenza with high morbidity but low mortality. The main clinical manifestations are only mild respiratory symptoms, reduced feed intake, egg production rate can drop from 90% to below 20%, or even complete production, and about 30% of commercial broilers can die, and it is very easy to mix infection with Escherichia coli. Seriously affect the production performance of poultry and bring serious economic losses to the poultry industry. More importantly, this subtype of AIV can pass through host barriers and infect mammals including humans, and it may also become a pandemic in humans, posing a serious threat to human health. At present, the H9N2 subtype is the main subtype of AIV existing in chick...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61K39/145A61K39/15A61K39/21A61P31/16A61P31/20
CPCA61K39/12A61K2039/552A61K2039/70C12N2710/10234C12N2720/12034C12N2740/11034C12N2760/16134
Inventor 张毓金谢秉超严悌昆敖艳华
Owner 广东渔跃生物技术有限公司
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