Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for purifying medium-chain and medium-long-chain mixed diglycerides

A technology of diglyceride and purification method, which is applied in the field of oil and fat, and can solve the problems such as the complexity of the crude product system of medium and long chain diglyceride

Active Publication Date: 2020-09-04
广东善百年特医食品有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medium and long chain diglyceride crude product system is complex and this method has not been prepared and purified by the medium and long chain and its mixed medium and long chain diglycerides before.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for purifying medium-chain and medium-long-chain mixed diglycerides
  • A method for purifying medium-chain and medium-long-chain mixed diglycerides
  • A method for purifying medium-chain and medium-long-chain mixed diglycerides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037](1) Novozyme 435 enzyme (Novozyme 435) that is equivalent to 5% of the total mass of the reactant is placed in a round-bottomed flask with magnetic stirring in an oil bath, then the oil bath heating switch is turned on to heat the oil bath, and the oil bath is heated. Heat the pot to 65°C; long-chain fatty acids mixed with monoglyceride and caprylic acid (monoglyceride / caprylic acid, 1:3 (mol / mol)) as reactants; after the temperature of the oil bath is preheated to 65°C, turn on the magnetic force Stir to 20rpm, stir the reactants evenly in the oil bath, initiate the enzyme-catalyzed reaction, and collect the crude product of lactonization in the round bottom flask; after the reaction is carried out for 40 minutes, turn off the magnetic stirring and the oil bath;

[0038] (2) molecular distillation is carried out on the obtained esterification crude product, and at distillation temperature is 160 ℃, and the rotating speed of scraping film electrode is 200rpm, and vacuum d...

Embodiment 2

[0044] This example is the same as Example 1 except the following technical features: caprylic acid monoglyceride described in step (4): anhydrous methanol carries out low-temperature high-speed centrifugal purification according to 1:3 (v / v) and 9% water, The temperature is 0°C, the rotation speed is 10000r / min, and the time is 5min.

[0045] Through the detection and calculation of the method described in Example 1, the content of medium-chain and medium-long-chain diglyceride mixture in the heavy phase of the crude product obtained after step (2) molecular distillation in this example is 56.3%; The content of the product reaches 61.3% after uniform stirring and crystallization; the final product is obtained after step (4) low-temperature high-speed centrifugation, and the content of the medium-chain and medium-long-chain diglyceride mixture is 86.6%.

Embodiment 3

[0047] This implementation example is the same as Example 1 except the following technical features: the raw material caprylic acid monoglyceride described in step (4): anhydrous methanol carries out low-temperature high-speed centrifugal purification according to 1:5 (v / v) and 9% water , temperature 0°C, rotation speed 10000r / min, time 5min.

[0048] Through the detection and calculation of the method described in Example 1, the content of the mixture of medium-chain and medium-long-chain diglycerides in the heavy phase of the crude product obtained after step (2) molecular distillation in this example is 56.3%; The content of the product reaches 61.3% after uniform stirring and crystallization; the final product is obtained after step (4) low-temperature high-speed centrifugation, and the content of the medium-chain and medium-long-chain diglyceride mixture is 84.9%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a purification method of medium-chain and medium- and long-chain mixed diglyceride. The method comprises the following steps of adding an enzyme and reactants long-chain fatty acid mixed monoglyceride and octylic acid to a reaction container, heating the enzyme and the reactants to a required reaction temperature, stirring the reactants evenly, initiating enzymic catalytic reaction and collecting an esterified crude product in the reaction container; carrying out molecular distillation on the obtained esterified crude product and obtaining a light phase and a heavy phase; taking the heavy phase, carrying out solvent fractionation by using low-temperature constant mixing crystallization, carrying out suction filtration to obtain liquid oil and solid lipid, collecting the liquid oil to obtain the medium-chain and medium- and long-chain mixed diglyceride with relatively high purity; and carrying out low-temperature high-speed centrifugation on the liquid oil, obtaining the liquid oil and the solid lipid again and collecting the liquid oil to obtain the high-purity medium-chain and medium- and long-chain mixed diglyceride. According to the method, the high-purity medium-chain and medium- and long-chain mixed diglyceride of over 87% can be obtained through low-temperature and high-speed centrifugation after fractionation.

Description

technical field [0001] The invention belongs to the field of oils and fats, and in particular relates to a method for purifying medium-chain and medium-long-chain mixed diglycerides. Background technique [0002] Diacylglycerol (DAG) is a product obtained by esterification of two molecules of fatty acid and one molecule of glycerol. Many studies have shown that diglycerides can prevent fat accumulation in the body, and have health functions such as regulating blood lipids, preventing arterial thrombosis, inhibiting visceral fat accumulation, relieving diabetic nephropathy, losing weight, and suppressing appetite. [0003] Diacylglycerol includes two isomers of 1,3-DAG and 1,2-DAG. About 70% of DAG in edible oils exists in the form of 1,3-DAG, while 1,2-DAG mainly exists in the middle of lipid metabolism in the product. Both medium-chain and medium-long-chain diglycerides are natural constituents of edible oils and fats and share the same advantages as diglycerides. The me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C07C67/48C07C69/003
CPCC07C67/48C12P7/6445C07C69/003
Inventor 汪勇李光辉陈佳子张震杨佳
Owner 广东善百年特医食品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com