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Method of promoting germination of Mallotus apelta seeds preserved at low temperature

A technology of low-temperature preservation and white-backed leaves, which is applied in the fields of seed and rhizome treatment, application, agriculture, etc., and can solve the problem that the seeds of white-backed leaves cannot germinate

Active Publication Date: 2017-12-01
江门市益林绿化工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problem in the prior art that after being stored at low temperature (-18°C to -20°C) for a period of time, the seeds of the white-backed leaves are almost completely unable to germinate, and to provide a simple and efficient way to germinate the seeds of the white-backed leaves after low-temperature storage. seed method

Method used

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  • Method of promoting germination of Mallotus apelta seeds preserved at low temperature
  • Method of promoting germination of Mallotus apelta seeds preserved at low temperature
  • Method of promoting germination of Mallotus apelta seeds preserved at low temperature

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Effect test

Embodiment 1

[0016] 1. Materials and methods:

[0017] 1.1 Research materials:

[0018] In October 2015, mature fresh Mallotus apelta seeds were collected in Guanyin Mountain, Yuanyang County, Honghe Prefecture, Yunnan Province. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0019] 1.2 Research methods:

[0020] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in step 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On July 20, 2016, the airtight container containing the seeds was placed in a refrigerator at -18°C to...

Embodiment 2

[0040] 1. Materials and methods:

[0041] 1.1 Research materials:

[0042]On September 27, 2014, Shi Chuan, Lingnan Township, Xiuning County, Huangshan City, Anhui Province collected mature fresh Mallotus apelta seeds. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0043] 1.2 Research methods:

[0044] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On September 14, 2015, the airtight container with the seeds was placed in a refrigerator at -18°C to -2...

Embodiment 3

[0057] 1. Materials and methods:

[0058] 1.1 Research materials:

[0059] On October 15, 2008, mature fresh Mallotus apelta seeds were collected in Mengdong River, Yongshun County, Xiangxi Autonomous Prefecture, Hunan Province. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0060] 1.2 Research methods:

[0061] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. In April 2012, the airtight container containing the seeds was placed in a refrigerator at -18...

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Abstract

The invention relates to a method of promoting germination of Mallotus apelta seeds preserved at low temperature. The method comprises the steps of taking out dry seeds preserved at low temperature between -18 DEG C and -20 DEG C and a closed container accommodating the seeds, standing for 24 hours in an environment with temperature of 15 DEG C and relative humidity of 15%, and taking the seeds out; putting the seeds in a cultivation box with temperature of 45 DEG C-55 DEG C, thermally treating for 4-8 hours, and then taking out the seeds; putting the seeds at room temperature, after the seeds recover to room temperature, washing with water to remove an external black loose oil-containing structure till the seeds are clean; absorbing water on the surfaces of the seeds with absorbent paper, and sowing the seeds in a 1% agar cultivation medium containing 200 mg / L gibberellin; putting the cultivation medium at 20 DEG C to enable the seeds to germinate, wherein the light intensity is 1000 lux, and the light period comprises 12 hours of light and 12 hours of darkness. The method promotes germination of Mallotus apelta seeds of different places of origin that are preserved for different time at low temperature. The problem that the Mallotus apelta seeds preserved at low temperature cannot germinate is solved. The method is easy to operate, and has remarkable effects when the seeds are thermally treated for 4-8 hours at 45 DEG C-55 DEG C.

Description

technical field [0001] The invention belongs to the technical field of plant seed preservation and germination, and in particular relates to a method for promoting the germination of alba dorsal seeds after storage at low temperature (-18°C to -20°C). Background technique [0002] Mallotus apelta (Lour.) Muell.Arg. is a deciduous shrub or small tree species of Euphorbiaceae (Mallotus Lour.). It often grows on the roadsides of flat hills in southern provinces and on barren and dry Guangshan or barren hills. It has strong adaptability and can be used to prevent soil loss and ecological restoration. The young leaves in spring can be mixed with other feed to feed pigs, and the leaves can be collected in winter to make compost. Stem bark is fibrous raw material, woven into sacks, used for blending or as raw material for wax paper and rayon. The roots and leaves of the white back leaf are used as medicine; the leaves have the effects of clearing away heat and dampness, relieving...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 胡枭剑亚吉东张桥蓉郭永杰刘成杨岚郭云刚杨湘云
Owner 江门市益林绿化工程有限公司
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