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Method for reducing heavy metal cadmium in rice flour through mixed fermentation by utilizing lactobacillus reuteri, lactobacillus fermentum and lactobacillus plantarum

A technology of Lactobacillus reuteri and Lactobacillus fermentum, which is applied in the field of heavy metal cadmium, can solve the problems of poor flavor of cadmium-removed rice products, poor cadmium-removal effect, and narrow application range, achieving significant social and economic benefits and high removal rate , The process is simple and reliable

Inactive Publication Date: 2017-12-01
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The research found that the cadmium content in rice can be reduced by physical methods such as soaking, hulling and rice milling, but the effect of removing cadmium is poor
Chemical methods can achieve better cadmium removal effect, but the cadmium-removed rice products obtained by acid or alkaline extraction have poor flavor, narrow application range, and may cause chemical residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take the rice with a cadmium content of 0.9778mg / kg, crush it to 40 mesh, add deionized water to the rice flour at the ratio of 1g rice flour to 4ml deionized water, and mix well to obtain rice slurry; add Lactobacillus reuteri , Lactobacillus fermentum and Lactobacillus plantarum suspensions are mixed at a volume ratio of 1:1:1, and then 2% of the inoculum weight is added to the rice slurry, and it is allowed to stand at a constant temperature of 35°C for 25h and filtered to obtain fermented rice flour. ; Wash the rice flour 4 times with deionized water of 2 times the weight of the fermented rice flour, filter by centrifugation (3000r / min, 8min.) to obtain the filter residue, and dry the filter residue at 45°C with hot air to a moisture content of 13.5%. For rice flour containing heavy metal cadmium, the content of heavy metal cadmium in the rice flour is 0.086mg / kg, and the reduction rate of cadmium in rice is 91.2%.

[0020] Control 1: The rice slurry does not incorpora...

Embodiment 2

[0030] Take the rice with a cadmium content of 0.9778mg / kg, crush it to 60 mesh, add deionized water to the rice flour at the ratio of 1g rice flour to 4ml deionized water, and mix well to obtain rice slurry; add Lactobacillus reuteri , Lactobacillus fermentum and Lactobacillus plantarum suspensions are mixed at a volume ratio of 1:1:1, and then 4% of the inoculum weight is inserted into the rice slurry, and then allowed to stand and ferment at 37°C for 16 hours, then filter to obtain fermented rice flour ; Wash the rice noodles twice with deionized water of 5 times the weight of fermented rice noodles, filter by centrifugation (3500r / min, 5min.) to obtain the filter residue, and dry the filter residue at 60℃ with hot air to a moisture content of 13.2%, which can be removed Rice flour of heavy metal cadmium. The heavy metal cadmium content in the rice flour is 0.093mg / kg, and the cadmium reduction rate in the rice is 90.5%.

Embodiment 3

[0032] Take rice with a cadmium content of 0.9778mg / kg, crush it to 50 mesh, add deionized water to the rice flour at the ratio of 1g rice flour to 4ml deionized water, and mix well to obtain rice slurry; add Lactobacillus reuteri , Lactobacillus fermentum and Lactobacillus plantarum suspensions are mixed at a volume ratio of 1:1:1, and then 3% of the inoculum weight is added to the rice slurry, left standing at a constant temperature of 36°C for 20h, filtered to obtain fermented rice flour ; Wash the rice noodles with 3 times the weight of fermented rice noodles with deionized water for 3 times, centrifuge (2500r / min, 10min.) to filter to obtain the filter residue, and dry the filter residue at 55°C with hot air to a moisture content of 13.4%, which is then removed Rice flour of heavy metal cadmium. The heavy metal cadmium content in the rice flour is 0.0812mg / kg, and the cadmium reduction rate in the rice is 91.7%.

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PUM

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Abstract

The invention discloses a method for reducing heavy metal cadmium in rice flour through mixed fermentation by utilizing lactobacillus reuteri, lactobacillus fermentum and lactobacillus plantarum. The method comprises the following steps: taking and pulverizing rice of which cadmium content exceeds the standard; adding deionized water into the rice of which the cadmium content exceeds the standard and uniformly mixing the rice with the deionized water to obtain a mixture; then adding mixed liquid of the lactobacillus reuteri, the lactobacillus fermentum and the lactobacillus plantarum into the mixture, allowing the obtained mixture to stand and ferment, and performing filtering to obtain fermented rice flour; then cleaning the fermented rice flour by using deionized water, and performing centrifugal filtration to obtain filter residues; and drying the obtained filter residues through hot air to obtain the rice flour in which the heavy metal cadmium is removed. According to the method disclosed by the invention, lactic acid bacteria with a heavy metal reduction effect is selected for mixed fermentation; and through effects of adsorption of groups on surfaces of bacteria bodies and leaching of metabolites of the bacteria bodies to cadmium, the heavy metal cadmium in the rice of which the cadmium content is 0.2mg / kg or above is reduced, and the removal rate of the heavy metal cadmium in the rice is up to 90 percent or above. The method is simple and reliable in process; and moreover, the content of the heavy metal cadmium in the rice can be effectively reduced, and the problem of utilization of the rice of which the cadmium content exceeds the standard can be effectively solved.

Description

Technical field [0001] The invention belongs to rice deep processing technology, and specifically relates to a method for reducing heavy metal cadmium in rice flour by using Lactobacillus reuteri, Lactobacillus fermentum and Lactobacillus plantarum mixed fermentation to reduce heavy metal cadmium. Background technique [0002] In recent years, the emergence of "cadmium rice" has caused widespread concern and anxiety about the safety of rice consumption. Today, excessive cadmium content in rice has become a major food safety issue that endangers people's health. Removal of cadmium from rice through processing technology is an emergency measure to solve the problem of rice utilization with cadmium exceeding the standard, which is beneficial to the healthy development of the rice industry and national food security. [0003] Studies have found that physical methods such as soaking, hulling and rice milling can reduce the cadmium content in rice, but the effect of cadmium removal is p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L7/104A23L5/20
CPCA23L7/104A23L5/28
Inventor 吴卫国陈瑶廖卢艳
Owner HUNAN AGRICULTURAL UNIV