Preparation method of acellular dermal matrix and prepared acellular dermal matrix

An acellular dermis, acellular technology, applied in medical science, tissue regeneration, bandages, etc., can solve the problems of acellular incomplete immunogenicity, low immunogenicity, etc., achieve good mechanical properties, low antigenicity, biological good compatibility

Inactive Publication Date: 2017-12-01
GUANGZHOU RAINHOME PHARM&TECH CO LTD
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing decellularized pig skin technology still has shortcomings such as incomplete decellularization and certain immunogenicity, which lead to certain limitations in clinical application. Therefore, there is an urgent need for a low-immunogenicity skin transplantable substitution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of acellular dermal matrix and prepared acellular dermal matrix
  • Preparation method of acellular dermal matrix and prepared acellular dermal matrix
  • Preparation method of acellular dermal matrix and prepared acellular dermal matrix

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0100] The present invention provides a method for preparing an acellular dermal matrix and the prepared acellular dermal matrix. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the method and application herein without departing from the content, spirit and scope of the present invention to realize and apply the present invention Invent technology.

[0101] The test materials and instruments used in the present invention are all common commercial products and can be purchased in the market.

[0102] The ol...

Embodiment 1

[0104] Example 1: Preparation method of heterogeneous acellular dermal matrix

[0105] a. Process for preparing split skin slices: choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, make the animal skin into slices with a thickness of 1.0mm with a skin-taking machine, and use a puncher to Fractured skin slices were made into sliced ​​skin slices of different shapes; soaked in 1‰ benzalkonium chloride solution for 30 minutes, degreased and sterilized;

[0106] b. Virus inactivation process: the virus inactivation solution composed of peracetic acid with a mass fraction of 1.0% and sodium chloride with a mass fraction of 3.0% was used to inactivate the tomographic skin for 45 minutes. The ratio is 1:5;

[0107] c, degreasing process: adopt the mixed degreasing agent that mass fraction 2.0% soda ash, mass fraction 2.0% caustic soda and mass fraction 0.5% surfactant form to carry out degreasing 30-60 minute, described surfactant is Ping Ping,...

Embodiment 2

[0114] Example 2: Preparation method of heterogeneous acellular dermal matrix

[0115] a, preparation process of split skin: choose Parmesan pigskin as the skin source, depilate and disinfect the skin area, use the skin machine to make the animal skin into a split skin with a thickness of 0.1mm, and punch it with a punch Fractured skin slices were made into sliced ​​skin slices of different shapes; soaked in 1‰ benzalkonium chloride solution for 20 minutes, degreased and sterilized;

[0116] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass fraction of 0.1% and sodium chloride with a mass fraction of 1.0% was used to inactivate the tomographic skin for 30 minutes. The ratio is 1:4;

[0117] C, degreasing process: adopt the mixed degreasing agent that mass fraction 1.0% soda ash, mass fraction 1.0% caustic soda and mass fraction 0.1% surfactant form to degrease 30 minutes, described surfactant is sodium lauryl aminopropionate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention relates to the technical field of tissue engineering, in particular to a preparation method of an acellular dermal matrix and the prepared acellular dermal matrix. By means of the provided preparation method of the acellular dermal matrix, cell ingredients can be cleanly removed, the antigenicity is low, the dermal collagen structures are reserved, the heterologous DNA content is very low, and good mechanical performance is obtained.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing an acellular dermal matrix and the prepared acellular dermal matrix. Background technique [0002] Skin is an important tissue and organ that covers and protects the body surface and internal organs. Skin defects and abnormalities are often caused by inflammation, ulcer trauma, burns, tumor surgery, and congenital deformities. Autologous skin grafts or skin flaps are often used to suppress such tissue defects, but this method not only creates new traumatic defects, but also often has limitations in donor sources. In addition, grafts often differ in color, texture, and function from the skin adjacent to the graft site. However, allogeneic skin or heterogeneous skin covering the wound is only temporary, and autologous skin still needs to be transplanted eventually. Moreover, heterogeneous or heterogeneous skins also have limited sources, are expe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60A61L26/00
CPCA61L26/0057A61L27/362A61L27/3687A61L27/60A61L2430/34
Inventor 王慧玲车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap