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Injectable hydrogel as well as preparation method and application thereof

A technology for injecting water and gel, applied in the fields of pharmaceutical formulation, medical science, surgery, etc., can solve the problems of high price of fibrin glue and poor resistance to enzymatic hydrolysis, and achieve the effect of remarkable anti-enzymatic hydrolysis ability and low cost.

Active Publication Date: 2017-12-01
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above deficiencies in the prior art, the object of the present invention is to provide an injectable hydrogel and its preparation method and application, aiming to solve the problems of the existing fibrin glue with high price and poor resistance to enzymatic hydrolysis

Method used

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  • Injectable hydrogel as well as preparation method and application thereof
  • Injectable hydrogel as well as preparation method and application thereof
  • Injectable hydrogel as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1. Synthesis of aldehydelated xanthan gum: prepare 0.6% (w / v) xanthan gum aqueous solution and 8% (w / v) NaIO 4 aqueous solution, then pour 80ml xanthan gum aqueous solution into a beaker and add 2ml NaIO dropwise 4 Aqueous solution (xanthan gum and NaIO 4 The molar ratio is 1:1.5); after that, the mixture was stirred for 3h in the dark; then 1ml of ethylene glycol was added to neutralize the unreacted NaIO 4 , the reaction was stirred for another 1 h, and the solution was purified with distilled water using a dialysis bag for 3 days, and the water was renewed 5 times a day during the dialysis; finally, the Xan-CHO product was freeze-dried in a freeze dryer, and then sealed at 4 °C Store in a plastic bag.

[0046] The degree of oxidation (proportion of oxidized xanthic acid repeating units) of Xan-CHO was determined by hydroxylamine hydrochloride titration. Dissolve 5ml xanthan gum CHO aqueous solution (0.2%w / v) in 15ml hydroxylamine hydrochloride solution (2.3%w / v), ...

Embodiment 2

[0059] 1, the synthesis of aldehydated xanthan gum: prepare 0.5% (w / v) xanthan gum aqueous solution and 5% (w / v) NaIO 4 aqueous solution, then pour the xanthan gum aqueous solution into a beaker and add NaIO dropwise 4 Aqueous solution (xanthan gum and NaIO 4 The molar ratio is 1:1); after that, the mixture was stirred for 2h in the dark; then 1ml of ethylene glycol was added to neutralize the unreacted NaIO 4 , the reaction was stirred for another 1 h, and the solution was purified with distilled water using a dialysis bag for 2 days, and the water was renewed 8 times a day during the dialysis; finally, the Xan-CHO product was freeze-dried in a freeze dryer, and then sealed at 4 °C Store in a plastic bag.

[0060] 2. Synthesis of carboxymethyl chitosan: Suspend 10g of chitosan in 50ml of isopropanol, then stir at room temperature, divide 20ml of 5mol / L NaOH aqueous solution into 5 parts, and add them to the stirring solution at intervals of 5min After that, the resulting s...

Embodiment 3

[0064] 1, the synthesis of aldehydated xanthan gum: prepare 2% (w / v) xanthan gum aqueous solution and 20% (w / v) NaIO 4 aqueous solution, then pour the xanthan gum aqueous solution into a beaker and add NaIO dropwise 4 Aqueous solution (xanthan gum and NaIO 4 The molar ratio is 1:3); Afterwards, the mixture was stirred for 4h in a dark room; Then 3ml of ethylene glycol was added to neutralize unreacted NaIO 4 , the reaction was stirred for another 2 h, and the solution was purified with distilled water using a dialysis bag for 4 days, and the water was renewed 5 times a day during the dialysis; finally, the Xan-CHO product was freeze-dried in a freeze dryer, and then sealed at 4 °C Store in a plastic bag.

[0065] 2. Synthesis of carboxymethyl chitosan: Suspend 20g of chitosan in 100ml of isopropanol, then stir at room temperature, divide 40ml of 15mol / L NaOH aqueous solution into 5 parts, and add them to the stirring solution at intervals of 5min After that, the resulting s...

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Abstract

The invention discloses an injectable hydrogel as well as a preparation method and an application thereof. The preparation method comprises the steps of suspending 10-20g of chitosan into 50-100ml of isopropyl alcohol; adding 20-40ml of 5-15mol / L NaOH water solution and stirring at room temperature for 20-40min, dropwise adding 20-30g of monochloro acetic acid, heating the water solution to 55-65 DEG C for 2-4h, filtering, washing and drying to obtain carboxymethyl chitosan; dropwise adding a sodium periodate water solution to a xanthan gum water solution at the molar ratio of xanthan gum to sodium periodate being 1: (1-3) and carrying out stirring reaction in a dark room for 2-4h; adding ethylene glycol and stirring for 1-2h, dialysizing and purifying a reaction solution to obtain aldehyde xanthan gum; and reacting the aldehyde xanthan gum with carboxymethyl chitosan to generate the injectable hydrogel. The injectable hydrogel synthesized after functional group modification by using natural polysaccharide is safe, nontoxic, low in cost and significant in enzymolysis resistance.

Description

technical field [0001] The invention relates to the field of injectable hydrogel, in particular to an injectable hydrogel and its preparation method and application. Background technique [0002] In clinical work, commercialized porcine-derived fibrin glue is usually used for closure of intestinal fistula, so that some patients with intestinal fistula can restore intestinal continuity through conservative treatment and avoid surgical treatment of intestinal fistula. However, the price of this fibrin glue is high due to its biological origin. In addition, this biological material has poor resistance to enzymatic hydrolysis, so that it is quickly and completely digested by intestinal juice during application, which makes some patients unsatisfied with the treatment effect. [0003] Therefore, the prior art still needs to be improved and developed. Contents of the invention [0004] In view of the above-mentioned deficiencies in the prior art, the object of the present inven...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08B37/08C08J3/075A61L31/14A61L31/04
CPCA61L31/042A61L31/14A61L31/145A61L2400/06C08B37/0003C08B37/003C08B37/0033C08J3/075C08J2305/00C08J2405/08C08L5/08C08L5/00
Inventor 任建安黄金健吴秀文陈国璞王革非顾国胜黎介寿
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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