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Method for co-preparing erythritol and intracellular fat by using illegal cooking oil as main raw material

A technology of the main raw material, erythritol, which is applied in the field of joint production of erythritol and intracellular oil, which can solve the problems of high price and high fermentation cost, and achieve the effects of reducing fermentation cost, increasing product yield, and simplifying product extraction process

Inactive Publication Date: 2017-12-05
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since erythritol fermentation mostly uses glucose as raw material, the fermentation cost is relatively high, and the price is relatively expensive, and the market price per ton reaches 4,000 US dollars

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] A method for preparing erythritol and intracellular oil by coproduction of waste oil, comprising: four steps of strain activation, seed cultivation, fermentation coproduction, product separation and purification;

[0019] 1) Streak the strain from the frozen glycerol tube onto the YPD plate, culture at 28°C, and activate the culture for 48 hours for seed inoculation and strain preservation. The strain is stored on the YPD slant;

[0020] 2) Shake flask fermentation conditions are: shake flask culture volume is 50 mL, inoculum size is 1×10 6 cells / L culture medium, the culture temperature is 26°C, the culture speed is 150 rpm, and the culture time is 3 days;

[0021] 3) The fermentation conditions of the fermenter are: fermentation system 5 L, fermentation temperature 28ºC, rotation speed 250 rpm, ventilation rate 1.0L / min / L culture volume. Bacterial inoculation amount is 1.0 ´ 10 8 Yeast cells / L culture medium. Fermentation time is 7 days;

[0022] 4) In the method ...

Embodiment 2

[0027] A method for preparing erythritol and intracellular oil by coproduction of waste oil, comprising: four steps of strain activation, seed cultivation, fermentation coproduction, product separation and purification;

[0028] 1) Streak the strain from the frozen glycerol tube onto the YPD plate, culture at 29°C, activate and culture for 52 hours, use for seed inoculation and strain preservation, and preserve the strain on the YPD slant;

[0029] 2) Shake flask fermentation conditions are: shake flask culture volume is 50 mL, inoculum size is 1×10 8 cells / L culture medium, the culture temperature is 30°C, the culture speed is 200 rpm, and the culture time is 7 days;

[0030] 3) The fermentation conditions of the fermenter are: fermentation system 5 L, fermentation temperature 28ºC, rotation speed 250 rpm, ventilation rate 1.0L / min / L culture volume. Bacterial inoculation amount is 1.0 ´ 108 Yeast cells / L culture medium. Fermentation time is 7 days;

[0031] 4) In the metho...

Embodiment 3

[0036] A method for preparing erythritol and intracellular oil by coproduction of waste oil, comprising: four steps of strain activation, seed cultivation, fermentation coproduction, product separation and purification;

[0037] 1) Streak the strain from the frozen glycerol tube onto the YPD plate, culture at 30°C, and activate for 72 hours for seed inoculation and strain preservation. The strain is preserved on the YPD slope;

[0038] 2) Shake flask fermentation conditions are: shake flask culture volume is 50 mL, inoculum size is 1×10 9 cells / L culture medium, the culture temperature is 36°C, the culture speed is 220 rpm, and the culture time is 10 days;

[0039] 3) The fermentation conditions of the fermenter are: fermentation system 5 L, fermentation temperature 28ºC, rotation speed 250 rpm, ventilation rate 1.0L / min / L culture volume. Bacterial inoculation amount is 1.0 ´ 10 8 Yeast cells / L culture medium. Fermentation time is 7 days;

[0040] 4) In the method of co-pr...

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Abstract

The invention relates to a method for preparing erythritol and intracellular fat by using illegal cooking oil as a main raw material. The high-efficiency production of the erythritol and intracellular fat is realized through a microbiological technology by using the illegal cooking oil as a sole carbon source in a culture medium and using Yarrowia lipolytica as a fermentation strain. The illegal cooking oil is used as the sole carbon source, and the addition amount of the illegal cooking oil in the medium is 10-100 g / L, and preferably 30 g / L. The present invention realizes the product self-separation mode. The method realizes the self-separation mode of the above products, can preliminarily separate the two products through centrifuging, simplifies the separation and the extraction of the products, and is suitable for large-scale industrial treatment modes. The method has the advantages of realization of high-efficiency conversion of the illegal cooking oil, remarkable increase of the yield of erythritol, obtaining of microbial fat, and remarkable improvement of the conversion values of the illegal cooking oil.

Description

technical field [0001] The invention relates to the technical field of waste oil utilization, in particular to the joint production of erythritol and intracellular oil by using waste oil as a main raw material. Background technique [0002] In recent years, China's "waste oil" incidents have emerged one after another. The situation about the waste oil flowing back to the dining table has repeatedly occurred, which makes people talk about the discoloration of waste oil, which has become a prominent food safety issue. The gutter oil incident in Taiwan in 2014 brought the issue of gutter oil to the forefront, and many companies were implicated. As the largest underground industrial chain, gutter oil is increasingly affecting our lives. With the development of science and technology and the correct guidance and encouragement of national policies, the utilization of waste oil has been put on the agenda and developed rapidly. [0003] Waste oil in the usual sense refers to the n...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12P7/64C12R1/645
CPCC12P7/18C12P7/64
Inventor 刘晓燕吕金顺徐继明许家兴夏军贺爱永张瞳
Owner HUAIYIN TEACHERS COLLEGE
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