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Tetramethylpyrazine-producing bacillus licheniformis and application thereof

A technology of Bacillus licheniformis and tetramethylpyrazine, applied in the field of fermentation engineering, can solve problems such as not being able to meet industrial production functions, and achieve far-reaching effects in favor of cell growth, improvement of healthy active substances in liquor, transformation and upgrading, and ecological manufacturing

Active Publication Date: 2017-12-08
ANHUI RUISIWEIER TECH
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  • Claims
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Problems solved by technology

[0003] Previously, the inventors used four strains of Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, and Bacillus lipolytica to carry out experiments on producing tetramethylpyrazine in single and mixed strain culture modes respectively. The results showed that although these strains were tested The yield of indoor tetramethylpyrazine is relatively high, but in the high-temperature Daqu culture mode, the content of tetramethylpyrazine is only 1 / 10 to 1 / 5 of that in the medium-temperature shelf Daqu culture mode, which is far from meeting the industrial production function of Daqu needs

Method used

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  • Tetramethylpyrazine-producing bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of Bacillus licheniformis functional bacteria culture fluid

[0033] Use an inoculation loop to pick 1-2 rings of Bacillus licheniformis GJBL055 strains stored on a slant and place them in 300-400mL liquid medium, culture them on a shaker at 120rpm at 37°C for 24 hours to obtain a first-grade seed liquid; Inoculate the inoculum of 10% of the inoculum into the fermenter, cultivate at 80rpm at 37°C for 24 hours, and obtain the second-grade seed solution; then inoculate the second-grade seed solution at 10% of the inoculation amount in the fermenter, and cultivate at 80rpm at 37°C for 24 hours to obtain the third-grade seed solution. The seed solution is the functional bacteria culture solution;

[0034] The 10% inoculation amount refers to the ratio of the volume of the primary or secondary seed solution to the volume of the fermenter.

[0035] Described liquid culture medium constitutes as follows by mass concentration:

[0036] Glucose 1w...

Embodiment 2

[0037] Embodiment 2: the optimization of the addition amount of Bacillus licheniformis functional bacteria culture solution

[0038] Add the functional bacteria culture solution obtained in Example 1 to the water storage tank at the rate of 0.1%, 0.5%, and 1% of the koji mass per room, and after mixing, add the koji-making material whole wheat flour, mix evenly, and press the shelf-style Daqu cultivation method.

[0039] When the different addition amount of table 1, tetramethylpyrazine output

[0040] Addition amount of functional bacteria solution

[0041] It can be seen from Table 1 that when the addition ratio of Bacillus licheniformis functional bacteria culture solution is 0.5%, the content of tetramethylpyrazine in Atrial Quercus is relatively high.

Embodiment 3

[0042] Example 3: Arrangement of functional frame Daqu

[0043] Add the functional bacteria culture solution obtained in Example 1 to the water storage tank at a rate of 0.5% of the koji mass per room, add the koji-making material whole wheat flour after mixing, fully mix the material by stepping on it, and control the moisture content to 36- 37%, to obtain bent billets, the thickness of each bent billet is controlled at 6.9-7.1cm; put the bent billets in a straight line on the curved frame, and place 32-34 curved billets on each shelf, and the distance between each curved frame 5-8 cm, there are three placement schemes in total, namely "five-layer shelf with six-layer curved", "five-layer shelf with five-layer curved (the bottom floor is not placed)", "four-layer shelf with five-layer curved", a total of Place 3 rows and 10 columns of curved racks.

[0044] Table 2 The content of tetramethylpyrazine in the atrial song when different arrangements of Daqu are arranged in the f...

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Abstract

The invention discloses tetramethylpyrazine-producing bacillus licheniformis and application thereof. The bacillus licheniformis is classified and named bacillus licheniformis GJBL055; the bacillus licheniformis is preserved in CCTCC (China Center for Type Culture Collection) on April 24th, 2017; the preservation code is CCTCC NO:M2017204; the preservation address is Wuhan University, China. The medium-temperature shelf culture mode is used; the bacillus licheniformis is inoculated into flat Daqu; through the pre-culture on functional microbes, the building of a shelf starter space model and the four-stage regulation and control on secret room temperatures, a Daqu manufacturing process for producing tetramethylpyrazine under the medium temperature condition is formed, so that the content of the tetramethylpyrazine in the Daqu is improved to 400mg / kg.

Description

technical field [0001] The invention relates to a tetramethylpyrazine-producing bacillus licheniformis and an application thereof, belonging to the technical field of fermentation engineering. Background technique [0002] "Qu is the bone of wine", and Daqu plays a vital role in the production of liquor. The purpose of the preparation is: the formation of multi-functional bacterial strain, multi-enzyme system and various aroma-generating substances. In the Daqu production process, bacteria and yeasts are first fermented to increase the temperature of the koji core, and the heat is dissipated to form a surface layer suitable for mold growth; then, with the dispersal of water, mold grows inside, and the curved surface gradually dries to form a closed layer. The biological heat generated by the multi-bacteria fermentation in Daqu is blocked, forming a higher "top fire temperature", and the aroma components are also produced at this time. In order to achieve the above goals, t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12G3/02C12R1/10
CPCC12G3/02C12N1/205C12R2001/10
Inventor 葛向阳何宏魁李晓欢张严
Owner ANHUI RUISIWEIER TECH
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