Methods and applications for increasing the content of active ingredients, consistency of chemical composition and/or therapeutic benefits of plants of the genus Larnia
A technology of Larnia and plants, which is applied in the field of processing and improvement of Larnia plants, and can solve the problems of excessive sulfur dioxide residue, easy mildew and qualitative change, and harm to human health, etc.
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Embodiment 1
[0093] Example 1: Effects of Different Processing Methods on the Active Components of Rabbittail Herb
[0094] 1-1. Metabolite analysis
[0095] In order to understand the influence of different processing methods on the harvested herbaceous rabbit tails, firstly, the dried rabbit tails of the group, the shade dried group, and the dried group were tested. 1 H-NMR metabolite analysis, the results are shown in figure 1 and Table 1. Among them, (A) is the drying group, the temperature is 22-28°C; (B) is the shade drying group, the temperature is 22-25°C; (C) is the drying group, the temperature is 40°C; Representative metabolite names are shown in Table 1.
[0096] As shown in Table 1, a total of 33 metabolites were identified in Rabbittail herb, which can be divided into primary and secondary metabolites, and their structures include amino acids, organic acids, sugars, nucleic acids, amines, triterpenoids ( triterpenoids), flavonoids (flavonoids), phenolic acids (phenolic ac...
Embodiment 2
[0119] Example 2: Effects of Different Drying Temperatures on the Active Components of Rabbittail Herb
[0120] 2-1. Metabolite analysis
[0121] The 40°C drying group, 55°C drying group, and 70°C drying group were treated with rabbit tail herbs respectively. 1 H-NMR metabolite analysis, the results are shown in image 3 . Wherein, the stronger the signal, the higher the content.
[0122] like image 3 As shown, compared with the drying group at 55°C or the drying group at 70°C, the content of amino acids, triterpenoids and flavonoids in the drying group at 40°C increased significantly, while the content of sugars decreased significantly.
[0123] 2-2. Metabolome analysis
[0124] Principal component analysis (PCA) was used to analyze the metabolites of the 40°C drying group, 55°C drying group, and 70°C drying group. The results are shown in Figure 4 .
[0125] Depend on Figure 4 It can be seen that the composition of the herbaceous herb in the 40°C drying group is s...
Embodiment 3
[0137] Embodiment 3: the comparison of different batches of Herba Rabbit's Tail Medicinal Materials
[0138] Merge the first batch of sun-dried group, shade-dried group, dried rabbit tail herbal medicine and the second batch of 40℃ dried group, 55℃ dried group, 70℃ dried rabbit tail herbal medicine 1 H-NMR data, and principal component analysis (PCA), in order to intuitively reflect the influence of different factors on the composition of the metabolites of the Herba Larbidis medicinal material, the results are shown in Figure 5 . Among them, the X-axis (PC1 axis, principal component 1) is to analyze different processing factors (including "40 ℃ (represents drying at 40 ℃)" and "non-40 ℃ (not 40 ℃)" shown on the PC1 axis other methods of dry processing)") on the consistency of the chemical composition of Rabbittail herb; the Y-axis (PC2 axis, principal component 2) is the analysis of different batches (including the "first batch" shown on the PC2 axis " and "Second Batch") ...
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