Lung cancer CTCs (Circulating Tumor Cells) capturing and activity detection and analysis methods and application thereof

Abstract

The invention provides lung cancer CTCs (Circulating Tumor Cells) capturing and activity detection and analysis methods and application thereof. On the basis of negative enriched lung cancer CTCs, by capturing on the lung cancer CTCs, the lung cancer CTCs are captured on a filter membrane; by carrying out combined detection on a nuclear morphometry and expression quantities of three specific mRNAs, the lung cancer CTCs are identified; suspected CTCs captured from each blood sample are subjected to cell nucleus size and morphological feature analysis; a TTF1 mRNA and a pan-CK mRNA are combined to identify whether the suspected CTCs are TTF1 positive lung cancer CTCs, so that according to a case whether MKI67 mRNA expression is positive, the suspected CTCs are classified as TTF1 positive lung cancer CTCs with the proliferative activity or TTF1 positive lung cancer CTCs without the proliferative activity. The detection method provided by the invention is high in sensitivity and high in specificity, the number of the TTF1 positive CTCs in peripheral blood of a lung cancer patient can be efficiently captured and counted; the activity of the TTF1 positive CTCs can be detected; whether each cell has the proliferative activity or not can be analyzed out.

Description

technical field [0001] The invention relates to the field of lung cancer liquid biopsy, in particular to a lung cancer circulating tumor cell capture and activity detection, analysis method and application thereof. Background technique [0002] Circulating tumor cells (CTCs) detection is one of the main contents in the field of tumor liquid biopsy, but the existing circulating tumor cell detection technology only relies on a certain characteristic of tumor cells to capture CTCs in the peripheral blood of cancer patients , simple counting, the shortcomings of these detection techniques are: on the one hand, the lack of specificity, the cells identified in peripheral blood may not be tumor cells, and there is currently no specific detection method for TTF1-positive lung cancer; The in-depth analysis of CTCs cannot obtain information on the proliferation activity of CTCs, and cannot identify which CTCs have the ability to metastasize and invade, and which CTCs have entered the ...

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Problems solved by technology

[0002] Circulating tumor cells (CTCs) detection is one of the main contents in the field of tumor liquid biopsy, but the existing circulating tumor cell detection technology only relies on a certain characteristic of tumor cells to capture CTCs in the peripheral blood of cancer patients , simple counting, the shortcomings of these detection techniques are: on the one hand, the lack of specificity, the cells identified in peripheral blood may not be tumor cells, and there is currently no specific detection method for TTF1-positive lung cancer; In-depth analysis of CTCs cannot obtain information on the proliferation activity of CTCs, and cannot identify which CTCs have the ability to metastasize and invade, and which CTCs have entered the stage of apop

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