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A method for breeding cytoplasmic male sterile hybrids resistant to clubroot

A technology of clubroot resistance and male sterility, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of less pollen, low-temperature yellowing nectaries, and low seed purity, achieving obvious advantages , high purity, simple and easy breeding method

Active Publication Date: 2019-07-23
HUAZHONG AGRI UNIV
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  • Application Information

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Problems solved by technology

The cytoplasmic male sterile types of cruciferous vegetables currently used in production are mainly rapeseed polCMS (polCMS) and radish cytoplasmic male sterile (ogu CMS), but rapeseed polCMS (polCMS) CMS) has the disadvantages of unstable sterility, low pollen quantity and low seed purity due to the influence of bad temperature; ogu CMS has the disadvantages of low-temperature yellowing and nectary degeneration, which have been improved at present

Method used

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  • A method for breeding cytoplasmic male sterile hybrids resistant to clubroot
  • A method for breeding cytoplasmic male sterile hybrids resistant to clubroot
  • A method for breeding cytoplasmic male sterile hybrids resistant to clubroot

Examples

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Embodiment 1

[0026] Example 1 Breeding of mustard tuberculosis-resistant cytoplasmic male sterile hybrids

[0027] The technical roadmap of the breeding method of this embodiment is as follows: figure 1 shown.

[0028] (1) Using the conventional mustard variety "Yong'an Xiaoye" (the seeds of this breeding material have been sold publicly before the application date) as the female parent, and the cabbage-resistant clubroot race 4 (which has been sold publicly) as the male parent, the The interspecific hybrid F1 was obtained by edge hybridization, and the conventional variety "Yong'an Xiaoye" was used as the recurrent parent. After backcrossing, the male parent line "Yong'an Xiaoye" of improved clubroot resistance was obtained.

[0029] (2) Use the mustard mustard resistant to clubroot variety "Improved Yong'an Shaoye" obtained in step (1) as the male parent, and the mustard hau cytoplasmic male sterile line as the female parent, and cross-breed to obtain mustard male sterile hybrids F1. ...

Embodiment 2

[0037] Example 2 Propagation of mustard mustard cytoplasmic male sterile line A and hybrid seed production

[0038] Under the isolation conditions of autumn sowing in Wuhan, Hubei, China (early September) or summer sowing in Lanzhou, Gansu, China (end of April every year), the mustard cytoplasmic male sterile line A was used as the female parent, and "6-170 (Lan W906)" was used as the male parent , according to the male parent: the female parent is 2:4 row ratio (row ratio) propagation of the mustard cytoplasmic male sterile line A, and other cultivation management is the same as the propagation of common mustard.

[0039] After breeding, the mustard mustard cytoplasmic male sterile line A was crossed with the clubroot-resistant mustard mustard inbred line "Yong'an Xiaoye" to obtain the mustard cytoplasmic male sterile hybrid variety.

[0040] in, figure 2 middle:

[0041] A: It refers to the mustard cytoplasmic male sterile line Lan W905, which shows that the mustard male st...

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Abstract

The invention provides a seed selection method of anti-club root tuber mustard cytoplasmic male sterile hybrid. The method comprises the following steps: 1) taking tuber mustard conventional variety as a female parent and taking cabbage anti-club root antigen as a male parent for hybridization to obtain an anti-club root improvement-type mustard tuber male parental line; and 2) taking a mustard tuber hau cytoplasmic male sterile line as the female parent and taking the anti-club root improvement-type mustard tuber male parental line for hybridization to obtain the anti-club root tuber mustard cytoplasmic male sterile hybrid; the mustard tuber hau cytoplasmic male sterile hybrid takes natural mustard-type rape cytoplasmic male sterile line 6-102A as the female parent, and the mustard tuber 6-170 is used as the male parent for hybridization. The anti-club root tuber mustard cytoplasmic male sterile hybrid has the characteristics of high purity and anti-club root disease, and overcomes the defects of anti-club root disease, successive cropping obstacle and variety degeneration existed in the current mustard tuber varieties.

Description

technical field [0001] The invention relates to the technical field of cruciferous vegetable breeding, in particular to a method for breeding cytoplasmic male sterile hybrids of mustard mustard resistant to clubroot. Background technique [0002] The heterosis in cruciferous vegetables is obvious, and the main ways to utilize heterosis are self-incompatibility and male sterility, among which cytoplasmic male sterility is an ideal pollination control system. Through the use of male sterile factors from different sources, the cytoplasmic male sterile lines of Chinese cabbage heart which are stable to the environment and ontogeny during the development process were selected and bred and utilized to prepare the first generation of hybrids with excellent comprehensive traits. The first-generation utilization of cruciferous vegetable hybrids has been widely carried out, especially in Asian countries, such as China, Japan, and South Korea. At present, self-incompatible lines or sel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 万正杰姚培杰邹瑞昌刘叁叁刘旭佳刘淑晶杨媛
Owner HUAZHONG AGRI UNIV
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