Plant tissue culture method using culture dish

Abstract

The invention belongs to the technical field of plant tissue culture and relates to a plant tissue culture method using a culture dish. The plant tissue culture method comprises five stages: the first stage is a stage at which aseptic treatment is performed and a culture solution enters a box body: pouring a water absorbing substrate into a tray with holes; the second stage is an aseptic culture stage: opening a front cover, and putting plant tissue materials on the water-absorbing substrate for culturing; the third stage is a stage of changing the culture liquid: opening a liquid leaking hole of the culture dish for draining the culture solution, closing the liquid leaking hole, opening the front cover, then pouring a new culture solution, and closing the cover; the fourth stage is a seedling establishing and rooting stage: cutting out seedlings grown into strains, changing into a rooting culture solution, replacing the tray with the holes with a fixed tray, and putting the seedlings grown into the strains into fixed sleeves; the fifth stage is a seedling hardening stage: opening a gland and an adjustable gland, and transplanting or transporting after about 3 days. Compared with a traditional plant culture method, the method disclosed by the invention has the advantages that cultured plants are shorter in period, and the seedlings are vigorous and high in survival rate.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture and relates to a plant tissue culture method using a culture device. Background technique [0002] In traditional plant tissue culture work, culture bottles and dishes are often used. The seedlings are placed in the culture bottle, and the space is small, which is not conducive to the growth of the seedlings. The leaves are placed in a petri dish. The moisture in the dish is heavy, which affects the growth of the callus. The traditional tissue culture process is too cumbersome and frequent, resulting in a lot of manual waste; the rooting medium is agar, which needs to be washed off when transplanting, which is easy to damage the root system, and it is not easy to transport and has a low survival rate. Summary of the invention [0003] In order to solve the above-mentioned problems, the present invention provides a method for organizing plants by using a culture device. [0004] The method o...

AI Technical Summary

Problems solved by technology

The traditional tissue culture process is too cumbersome, with many times, resulting in a lot of labor waste; the rooting medium is agar, and the agar needs to be washed off when transplanting, which is easy to damage the root system, and it is not easy to transport, and the survival rate is low

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