Reusable light optical fiber electrode as well as making method and embedding method thereof

Abstract

The invention provides a reusable light optical fiber electrode as well as a making method and an embedding method thereof. A laser diode is connected to one end of an optical fiber; a neural signal connector is welded on a PCB; the neural signal connector is electrically connected to the PCB; the PCB is welded on a micro thruster; an iron sheet is perpendicularly fixed to the micro thruster; a capillary guiding tube is stuck on the iron sheet; a tetrode is inserted into the capillary guiding tube; one end of the tetrode protrudes out of the capillary guiding tube and the other end of the tetrode is inserted into a hole of the PCB; a laser diode-optical fiber complex and tetrode electrodes are cured by virtue of glue; one end of a connecting rod is fixed to the micro thruster; and the other end of the connecting rod is fixed to a laser diode base. The laser diode is drawn and controlled by virtue of an extra-fine electric wire, without inserting or removing an optical fiber or an optical fiber sleeve, so that stable multi-channel recording and synchronous multi-modal photic stimulation control can be conducted on two or more encephalic regions of a freely moving mouse easily. The reusable light optical fiber electrode is small in size, light in weight and reusable, so that using cost can be greatly reduced.

Description

technical field [0001] The invention relates to the technical field of optical fiber electrodes, in particular to a reusable portable optical fiber electrode, a manufacturing method and an embedding method thereof. Background technique [0002] Optogenetics is currently the most common method for manipulating the activity of neurons and neural circuits. By coupling with the electrode array and using multi-channel recording technology, the effectiveness of specific wavelength light in controlling neuron activity can be verified in vivo. Existing fiber optic electrodes often have the following disadvantages: (1) Before light stimulation, the optical fiber needs to be inserted into the ceramic sleeve. Since one end of the ceramic sleeve is connected to the recording electrode, it is inevitable that the tip of the recording electrode will be displaced, thereby reducing the stability of neuron activity recording. (2) The volume is large, so it can only record and control a sing...

AI Technical Summary

Problems solved by technology

Since one end of the ceramic sleeve is connected to the recording electrode, it will inevitably cause the tip of the recording electrode to shift, thereby reducing the stability of neuron activity recording

(2) The volume is large, so it can only record and control a single brain area in mice, and simply verify the effect of light control, but cannot record and control activities in two or more brain areas at the same time

(3) It is difficult to control multi-modal light, and the electrode tip can only export light of one color

(4) Due to the brittleness of the electrode, the fiber optic electrode is easily damaged during use, which has high requirements for the professionalism and precision of the user's operation; (5) It is difficult to be recycled and used

Fiber optic electrodes are expensive and cannot be reused, which will greatly increase the cost of use

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