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Preparation method of high-purity chlorogenic acid

A chlorogenic acid and high-purity technology is applied in the field of preparation of high-purity chlorogenic acid to achieve the effects of low cost, improved purification effect, simple and safe process

Inactive Publication Date: 2017-12-22
BINHAI IND TECH RES INST OF ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when using HPD series macroporous adsorption resin to purify chlorogenic acid, the purity is usually below 40%, and often only reaches about 30%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 100g Yuanbao maple leaves are extracted with 1000ml of 50% ethanol at a pH value of about 4 and a temperature of 50°C under reflux and shaking for 2 times, each time for 3 hours, and the resulting extract is 1760ml, adjusted to pH=3-4; add 500ml Mix macroporous resin for adsorption, wherein the volume ratio of macroporous resin is HPD400:XAD-5=1:1; sample loading speed is 12ml / min, then elute with 75% ethanol with a column volume of 3BV, collect the eluate, concentrate and dry , the yield of the obtained dry powder chlorogenic acid was 4.15%, and the purity reached 92.3%.

Embodiment 2

[0018] 150g Yuanbao maple leaves were extracted with 1600ml of 50% ethanol at a pH value of about 4 and a temperature of 60°C under reflux and shaking for 2 times, each extraction was 3 hours, and the obtained extract was 2900ml, adjusted to pH=3-4; add 750ml Mix macroporous resin for adsorption, wherein the volume ratio of macroporous resin is HPD400:XAD-5=1:3; the sample loading speed is 15ml / min, and then eluted with 75% ethanol with a column volume of 3BV, the eluate is collected, concentrated and dried , the yield of the obtained dry powder chlorogenic acid was 4.65%, and the purity was 91.8%.

Embodiment 3

[0020] 200g Yuanbao maple leaves are extracted with 2400ml of 50% ethanol at a pH value of about 4 and a temperature of 60°C under reflux and shaking for 2 times, each time for 3 hours, and the resulting extract is 4410ml, adjusted to pH=3-4; add 1000ml Mix macroporous resin for adsorption, wherein the ratio of macroporous resin is HPD400:XAD-5=1:5; sample loading speed is 18ml / min, then elute with 75% ethanol with column volume 3BV, collect eluate, concentrate and dry , the yield of the obtained dry powder chlorogenic acid was 5.11%, and the purity was 90.8%.

[0021] Control test: Example 2 is used as the test group; the macroporous resin in Example 2 is replaced by XAD-5 and used alone, other steps and parameters remain unchanged, as the control group 1, the yield of the obtained dry powder chlorogenic acid is 4.75% , the purity was 44.8%; the macroporous resin in Example 2 was replaced by HPD400 and used alone, other steps and parameters were unchanged, as the control grou...

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Abstract

The invention relates to a preparation method of high-purity chlorogenic acid, which includes the steps of: uniformly mixing XAD-series macroporous adsorption resin and HPD-series macroporous adsorption resin according to volume ratio of 1-5:1, packing the mixed resin in a column, purifying a to-be-purified chlorogenic acid solution through the mixture adsorption column, and performing elution to obtain the high-purity chlorogenic acid. The method overcomes defect of incomplete impurity removal due to adsorption through single resin; the purity of chlorogenic acid can reach more than 90%; the method is simple and safe, is low in cost and is beneficial to large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and purifying chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid. Background technique [0002] Chlorogenic acid is a polyphenolic compound with the molecular formula C 16 h 18 o 9 ,complex structure. Due to its diverse pharmacological functions, it has attracted widespread attention at home and abroad, and the market demand is very large; chlorogenic acid is widely distributed in the plant kingdom, but its content is low. At present, artificial synthesis has not been realized at home and abroad, so in-depth research on chlorogenic acid The advanced extraction and purification process is of great significance to the development of a series of chlorogenic acid products. [0003] At present, XAD series macroporous adsorption resins are mostly used for the purification of chlorogenic acid, and the purity of the obtained chlorogenic acid can reach about 40%. However, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/732
CPCC07C67/48C07C67/56C07C69/732
Inventor 刘雪松栾连军蔺海浪谈满良杨英士
Owner BINHAI IND TECH RES INST OF ZHEJIANG UNIV