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Biomarker for diagnosing kawasaki diseases and kit and application

A biomarker and biomarker technology, applied in the field of molecular biology, can solve rare problems and achieve the effects of reducing false positives, being easy to operate, and improving the detection rate

Inactive Publication Date: 2017-12-22
SUZHOU ZHONGXIN BIOTECH
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  • Claims
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AI Technical Summary

Problems solved by technology

Platelets also play a role in signal exchange and transfer transmission in the immune response. Since Kawasaki disease is a systemic immune disease, the study of platelet RNA has a theoretical basis and practical significance for the early diagnosis of Kawasaki disease. There are very few research reports on

Method used

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  • Biomarker for diagnosing kawasaki diseases and kit and application
  • Biomarker for diagnosing kawasaki diseases and kit and application
  • Biomarker for diagnosing kawasaki diseases and kit and application

Examples

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Embodiment 1

[0055] 1. Screening of biomarkers for the diagnosis of Kawasaki disease

[0056] The screening method is as follows:

[0057] (1) 1 mL of blood was collected from 20 children with other febrile diseases and 20 children with KD using EDTA anticoagulant tubes, stored in a refrigerator at 4°C, and the platelets were separated by centrifugation within 48 hours, and the separated platelets were washed with phosphate buffer for 2 After the second time, if not used immediately, it can be stored in liquid nitrogen for a long time;

[0058] (2) According to the operating instructions of the miVana miRNA Isolation Kit kit, extract RNA from platelets, and use Qubit and Nanodrop to determine its concentration and purity;

[0059] (3) Use NEBNext miRNA Library PrepKit to construct a miRNA library of 100ng of extracted RNA, and cut the gel to recover fragments of 140bp-350bp. The library construction process fully complies with the instructions of NEBNext miRNA LibraryPrepKit;

[0060] (4...

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Abstract

The invention discloses a biomarker for diagnosing kawasaki diseases and a kit and application. The biomarker for diagnosing kawasaki diseases comprises 11 miRNAs such as miR-223-3p, miR-182-5p, miR-183-5p, miR-378-3p, miR-30c-5p, miR-148a-3p, miR-27a-3p, miR-145 miR-140-3p, miR-125a-5p and miR-30e-3p. The kit comprises primers for performing PCR (Polymerase Chain Reaction) detection on the 11 miRNAs. The content of the 11 miRNAs can be acquired by using the kit with corresponding PCR kits, and the risk of kawasaki diseases can be calculated through CT (Computed Tomography) values. The biomarker has the advantages of high sensitivity, good stability and the like, and the detection rate of kawasaki diseases in early diagnosis is increased.

Description

technical field [0001] The present invention relates to the technical field of molecular biology, more specifically, to the technical field of DNA, in particular to a biomarker and kit for diagnosing Kawasaki disease and its application. Background technique [0002] Kawasaki disease (KD) was first reported by Tomisaku Kawasaki, Japan in 1967. It was once called mucocutaneous lymph node syndrome (MCLS). Vasculitis. Its main age of onset is 6 months to 4 years old, characterized by extensive small and medium-sized vasculitis, with the most serious damage to the cardiovascular system, mainly involving coronary arteries, about 15% to 25% of untreated children eventually develop coronary artery disease Arterial damage, making it the most common cause of acquired heart disease in children in developed countries. Studies have shown that long-term vascular endothelial dysfunction exists in both the acute and recovery stages of KD, which is a risk factor for coronary atheroscleros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 魏冬凯丁国徽吴洁浦宇
Owner SUZHOU ZHONGXIN BIOTECH
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