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Wedelia trilobata anti-tumor extract and preparation and application methods thereof

An extract and anti-tumor technology, which is applied in the field of natural herbal medicines, can solve the problems of separation of chemical components from unseen and no anti-tumor effective parts, and achieve good anti-tumor activity, low cost, and simple process.

Active Publication Date: 2017-12-29
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there are no patents related to Wedelia chinensis in the application of tumor therapy, and there is no report on the isolation of effective anti-tumor parts and further chemical components of Wedelia chinensis

Method used

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  • Wedelia trilobata anti-tumor extract and preparation and application methods thereof
  • Wedelia trilobata anti-tumor extract and preparation and application methods thereof
  • Wedelia trilobata anti-tumor extract and preparation and application methods thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0026] The preparation method step of South American Wedelia chrysanthemum extract is as follows:

[0027] 1) Add 85-95% ethanol or methanol aqueous solution to the pulverized Wedelia chrysanthemum medicinal material (passed through a 20-80 mesh sieve), soak at room temperature, heat or ultrasonically extract, the extraction time is 60 minutes to 7 days, and the solid-liquid ratio 1:1~1:10;

[0028] 2) Filtrate the extract of Wedelia chrysanthemum medicinal materials, recover under reduced pressure to obtain the total extract, add water to suspend, add water to suspend, sequentially extract with petroleum ether and ethyl acetate, and recover under reduced pressure to obtain the petroleum ether part and the ethyl acetate part respectively and water soluble part;

[0029] 3) The petroleum ether part was chromatographed on a silica gel column, first eluted with petroleum ether or n-hexane to remove grease, then eluted with ethyl acetate, and concentrated in vacuo to obtain Wedel...

example 1

[0032]Example 1 Preparation of effective parts of Wedelia chrysanthemum flowers

[0033] South America Wedelia chrysanthemum (10Kg), at room temperature adopts 95% ethanol at room temperature to soak 3 times, 7 days / time, combine extracts, concentrate under reduced pressure to obtain total extract (1.2Kg), add water to suspend and disperse, respectively use sherwood oil, chloroform, Ethyl acetate and n-butanol were extracted to obtain a petroleum ether fraction (200 g), an ethyl acetate fraction (380 g) and a water-soluble fraction.

[0034] The active site after partial degreasing of petroleum ether was subjected to repeated silica gel and Sephdex LH-20 column chromatography to obtain 1 eucalyptane-type sesquiterpene lactone and 5 ent-kaurane-type diterpenes compounds, 2 oleanane-type triterpenes, 1 cycloaltinane-type triterpene, 1 sterol compound, 2 dipeptide compounds and 1 caffeate compound, respectively petroleum ether: acetone (15:1), petroleum ether: ethyl acetate (20:...

Embodiment 2

[0038] Example 2 Study on Antitumor Activity of Wedelia chrysanthemum Flower Extract

[0039] 1. Experimental method: Four kinds of tumor cell lines SPCA-1, SGC-7901, K562, BEL-7402 were used in RPMI-1640 medium containing 10% calf serum at 37°C, 5% CO 2 cultured in an incubator. The MTT method was used to carry out the cell proliferation inhibition test. The main operation was to take the tumor cell lines in the logarithmic growth phase, digest them with 0.25% trypsin, and adjust the RPMI-1640 culture solution with 10% newborn calf serum to 5×10 4 pcs / ml -1 The cell suspension was inoculated in a 96-well plate with 180 μL per well. At 37°C, 5% CO 2 Cultivate for 8-10 hours under saturated humidity conditions, and when they adhere to the wall, add sample solution prepared with PBS to each well so that the final concentrations of the samples are 1, 10, and 100 μg / mL, respectively. For each concentration, 3 wells were paralleled, and after continuing to culture for 44 hours,...

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Abstract

The invention discloses a wedelia trilobata anti-tumor extract and preparation and application methods thereof. The wedelia trilobata anti-tumor extract is prepared by adding solvent into wedelia trilobata powder for extraction, filtering and decompression-concentrating extracted liquor to obtain a total extract, mixing the total extract with water into a suspension, performing extraction sequentially through petroleum ether and ethyl acetate and performing decompressed recovery to obtain a petroleum ether solution, an ethyl acetate solution and a aqueous solution, wherein the petroleum ether solution contains one eudesmene type sesquiterpene lactone, five enantiotopic kaurane type diterpenoid compounds, two oleanane type triterpenes, one cycloartane type triterpene, one sterol compound, two dipeptide compounds and one caffeate compound. The prepared wedelia trilobata anti-tumor extract is high in yield, simple in technical process, low in cost and applicable to large-scale preparation, and meanwhile, has broad-spectrum anti-tumor activity and can serve as an active component applied to tumor control and treatment drugs.

Description

technical field [0001] The invention belongs to the field of natural herbal medicines, and in particular relates to an anti-tumor extract from Wedelia chrysanthemum flowers and its preparation method and application. Background technique [0002] South American Wedelia (Wedelia trilobata), also known as three-lobed (leaf) Wedelia, is a perennial herbaceous plant belonging to the genus Wedelia in the Compositae family. It is native to central and southern tropical America. The leaves or aerial parts of the plant are used in traditional medicine in the Caribbean and Central America to treat back pain, muscle spasms, rheumatism, stubborn wounds, ulcers, swelling, and joint pain. It was first introduced to Hong Kong in the 1870s to replace the traditional Chinese medicine Wedelia, mainly used to treat diphtheria, whooping cough, dysentery, hemorrhoids, and bruises. Nowadays, on the roadsides, fields, ditches or wet grasslands widely distributed in Guangdong, Guangxi, Fujian and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/92C07C69/732C07C62/34C07C62/38C07C237/20C07C62/32C07C51/42C07C67/48C07J63/00C07J9/00A61P35/00A61K36/28
CPCA61K36/28A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55C07C51/42C07C67/48C07C231/24C07D307/92C07J9/00C07J63/008C07C69/732C07C62/34C07C62/38C07C237/20C07C62/32
Inventor 惠阳陈文豪陈光英宋小平韩长日戴春燕
Owner HAINAN NORMAL UNIV
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