Low-temperature mutation breeding method for tulips

Abstract

The invention discloses a low-temperature mutation breeding method for tulips. The low-temperature mutation breeding method is characterized by including the following steps of seed pretreatment, wherein tulip seeds are soaked in a 180-ppm sodium hypochlorite solution and irradiated by an ultraviolet lamp to be sterilized; high and low temperature chemical mutagenesis, wherein a mixed chemical mutagen is prepared from diethyl sulfate, sodium nitrite and hydroxylamine and used for soaking the tulip seeds for mutagenesis, and the tulip seeds are placed in a calorstat with alternating high temperature and low temperature; centrifugal treatment, wherein the tulip seeds obtained after being soaked in a colchicines solution with the concentration of 0.05-0.07% are placed in a centrifugal at therotating speed of 4100-4300 r / min, and the seeds mutate in a centrifugal superheavy state; low-temperature culture, wherein the germination stage of tulips is under low temperature, the germination growth cycle is prolonged, and more mutation changes are obtained; later stage cultivation, wherein the tulip seeds with stable characters are obtained for mass breeding reproduction.

Description

technical field [0001] The invention belongs to the technical field of flower planting, and in particular relates to a low-temperature mutation breeding method for tulips. Background technique [0002] Tulip, a herbaceous plant of the genus Tulip in the family Liliaceae, is native to the Mediterranean coast, Central Asia, Turkey and other places. It has a wide planting area in my country and a long history of planting. Tulip flowers are strong and straight, with elegant and beautiful leaves, and have high ornamental value. , and has the medicinal value of treating dampness, chest fullness, nausea and abdominal pain. There are various varieties of tulips and colorful flowers. Through continuous cultivation of new varieties, the quality of tulips is more colorful. For the breeding of new tulip varieties, methods such as cross-pollination, chemical mutagenesis, tissue and seed culture and irradiation are generally used to obtain tulip mutant plants. Tulip hybrid pollination is...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at existing problems: tulip hybrid pollination breeding, simple operation, low technical requirements, but long cultivation period, low mutation rate, little difference between new varieties and existing varieties; chemical mutagenesis, single mutagen and The use of influencing factors, the seeds are in a relatively stable environment, and the self-system adjustment method is relatively stable, which makes the level of chemical mutation mutation relatively low, and the efficiency of obtaining new tulip varieties is not high; tissue and seed irradiation seedlings require high technology and equipment requirements and complicated operations , while radiation is harmful to the human body; mutation breeding mostly occurs in the germination and growth stage of seeds, but under normal circumstances, the germination and growth of seeds is faster, and the accumulation of variation factors is limited by time, so it is difficult to express