Preparation method of flaxseed meal polypeptide

A technology of linseed meal and polypeptide liquid, which is applied in the field of preparation of linseed meal polypeptide, can solve the problems of high cost, long process time, destruction of protein or amino acid structure and properties, etc., and achieves short time, good application prospect and high extraction rate high effect

Active Publication Date: 2018-01-09
无锡秋可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, relevant researchers obtain polypeptides from flaxseed meal, and the preparation of flaxseed meal protein is carried out in the middle. However, the commonly used

Method used

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  • Preparation method of flaxseed meal polypeptide

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Experimental program
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Effect test

Embodiment 1

[0034] A preparation method of linseed meal polypeptide, said preparation method comprising the steps of:

[0035] (1) Pulverize the shelled linseed meal with a pulverizer, then cross a 40-mesh sieve to remove impurities to obtain pulverized material;

[0036] (2) Mix the ground linseed meal with the mixed solvent n-hexane-isopropanol-NaOH (the mass ratio of the ground linseed meal to the mixed solvent is 1:6, n-hexane:isopropanol:NaOH=2:7 : 1 (volume ratio), the massfraction of NaOH solution is 5%), utilize ultrasonic (power is 400w) auxiliary leaching 4h, centrifugal (4000r / min, 10min) carries out solid-liquid separation, obtains solid wet meal;

[0037] (3) Put the solid wet meal prepared in step (2) into an oven for drying at a temperature of 55° C. to remove the organic solvent remaining in the sample;

[0038] (4) Add water to the dried linseed meal in step (3), mix and adjust to a mass concentration of 10%, finely pulverize (power 45Hz) and colloid mill to obtain a lin...

Embodiment 2

[0046] (1) Pulverize the shelled linseed meal with a pulverizer, then cross a 50-mesh sieve to remove impurities to obtain pulverized material;

[0047] (2) Mix the ground linseed meal with the mixed solvent n-hexane-isopropanol-NaOH (the mass ratio of the ground linseed meal to the mixed solvent is 1:7, n-hexane:isopropanol:NaOH=3:6 : 1 (volume ratio), the mass fraction of NaOH solution is 7%), utilize ultrasonic (power is 500w) auxiliary leaching 3h, centrifuge (4500r / min, 7min) carry out solid-liquid separation, get solid wet meal;

[0048] (3) Put the solid wet meal prepared in step (2) into an oven to dry at a temperature of 60° C. to remove the organic solvent remaining in the sample;

[0049] (4) Add water to the dried linseed meal in step (3), mix and adjust to a mass concentration of 15%, finely pulverize (power 35Hz), and colloid mill to obtain a mixed liquor of linseed meal;

[0050] (5) Enzymolyze the linseed meal mixture by using a two-step enzymolysis method, ad...

Embodiment 3

[0057] (1) Pulverize the shelled linseed meal with a pulverizer, then cross a 60-mesh sieve to remove impurities to obtain pulverized material;

[0058] (2) Mix the ground linseed meal with the mixed solvent n-hexane-isopropanol-NaOH (the mass ratio of the ground linseed meal to the mixed solvent is 1:8, n-hexane:isopropanol:NaOH=4:5 : 1 (volume ratio), the massfraction of NaOH solution is 10%), utilize ultrasonic (power is 600w) auxiliary leaching 2h, centrifuge (5000r / min, 5min) carry out solid-liquid separation, get solid wet meal;

[0059] (3) Put the solid wet meal prepared in step (2) into an oven to dry at a temperature of 60° C. to remove the organic solvent remaining in the sample;

[0060] (4) Add water to the dried linseed meal in step (3), mix and adjust to a mass concentration of 20%, finely pulverize (power 40Hz) and colloid mill to obtain a mixed liquor of linseed meal;

[0061] (5) Enzymolyze the linseed meal mixture by using two-step enzymolysis method, adjus...

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Abstract

The invention discloses a preparation method of flaxseed meal polypeptide. The preparation method comprises the following steps: (1), crushing and sieving, so as to obtain crushed materials; (2), performing ultrasound-assisted extraction, so as to obtain solid wet meal; (3), drying and removing a solvent; (4), adding water to prepare flaxseed meal mixed liquor; (5), performing enzymolysis on the flaxseed meal mixed liquor according to a two-step enzymolysis method; (6), performing sterilization on enzymatic hydrolysate via UHT; (7), performing liquid solid separation on the enzymatic hydrolysate subjected to sterilization, so as to obtain flaxseed meal polypeptide solution; (8), performing vacuum concentration, so as to obtain polypeptide concentrated solution; (9), drying the polypeptideconcentrated solution, so as to obtain flaxseed meal polypeptide powder. The method has the advantages that flaxseed meal is subjected to processes, such as impurity removal and detoxification, and then is directly subjected to enzymolysis, so that the polypeptide solution is obtained, the technology is simplified, the cost is reduced, the obtained polypeptide powder has high antioxidant activity,and a technical gap is filled up.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of linseed meal polypeptide. Background technique [0002] Flaxseed meal is the residue of flaxseed after solvent extraction or mechanical pressing to extract oil. It is generally treated as animal feed or waste, and its utilization rate is low, and its high added value has not been realized. However, its protein content is 32% to 49%, rich in amino acids, and it is one of the few varieties of vegetable protein that can replace animal protein. However, after linseed oil is produced, most of the cyanogenic glycosides remain in the linseed meal, and some of them have been hydrolyzed to hydrocyanic acid, which directly limits the utilization of the linseed meal, so it is particularly important to detoxify the linseed meal. [0003] At present, the protein intake of Chinese residents is mainly animal protein and grain protein. High animal protein intake ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 于秋生尹义敏陈天祥陈林李珍妮冯伟朱熹
Owner 无锡秋可生物科技有限公司
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