Method for detecting jinnan cattle IGFIR gene CNV mark and application of method

A technology of BTF3-CNV and gene, which is applied in the field of detection of IGF1R gene copy number variation of Jinnan cattle, can solve the problems of tumor generation, growth inhibition, and affecting the physiological function of IGF1R, and achieve the effect of simple operation, accurate and reliable method

Active Publication Date: 2018-01-09
NORTHWEST A & F UNIV
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Problems solved by technology

At present, it is revealed that the variation of IGF1R genome will affect the physiological function of IGF1R, and even lead to growth arrest, tumor and other diseases
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  • Method for detecting jinnan cattle IGFIR gene CNV mark and application of method
  • Method for detecting jinnan cattle IGFIR gene CNV mark and application of method
  • Method for detecting jinnan cattle IGFIR gene CNV mark and application of method

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0034] The present invention utilizes real-time fluorescent quantitative PCR to detect the copy number variation of Jinnan cattle IGF1R gene and is used for molecular breeding, comprising the following steps:

[0035] (1) According to the bovine IGF1R gene sequence in the NCBI database, use Primer 5.0 software to design primers, and use ordinary PCR to detect primers;

[0036] (2) Using real-time fluorescence quantitative PCR (qPCR) technology to detect the copy number variation of the candidate loci in the population, and to screen out CNV markers related to the growth traits of Jinnan cattle;

[0037] (3) Use SPSS 23.0 software to conduct association analysis between the type of copy number variation and the growth traits of cattle;

[0038] (4) Breeding of Jinnan cattle with excellent growth traits according to the type of copy number variation. ...

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Abstract

The invention discloses a method for detecting a jinnan cattle IGFIR gene CNV mark and application of the method. The method comprises the following steps: taking an ear tissue whole-genome DNA of a jinnan cattle as a template; utilizing a pair of specific primers IGF1R-CNV to amplify a copy number variable region of the cattle IGF1R gene; utilizing a pair of specific primers BTF3-CNV to amplify acattle BTF3 gene segment as a control group; and utilizing a 2-delta Ct method to calculate copy number of the individual. The method provided by the invention lays foundation for establishing correlation between the jinnan cattle IGF1R gene copy number and growth traits; copy number variation and the growth traits are subjected to related analysis, so that jinnan cattle molecular marker-assistedselection work is facilitated. The method is simple, is quick and is convenient to popularize and apply.

Description

technical field [0001] The invention belongs to the field of molecular genetics research, and in particular relates to a method for detecting the copy number variation of Jinnan cattle IGF1R gene. The method utilizes real-time quantitative PCR technology, uses genomic DNA as a template, and takes BTF3 gene as a reference, according to 2*2 -ΔCt value to determine whether an individual's copy number is inserted or deleted. Background technique [0002] With the rapid development of related disciplines such as genomics and bioinformatics, various biological mechanisms of animal growth and development have been revealed. Now people continue to improve the quality and quantity of beef to promote the process of cattle industrialization, so as to meet people's increasing demand for beef. Body size traits and beef quality are jointly regulated by multiple genes. It is now considered that molecular marker-assisted selection is effective for the development of animal breeding by inc...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈宏马懿磊蔡含芳白跃宇马云雷初朝蓝贤勇黄永震
Owner NORTHWEST A & F UNIV
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