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Application of rela gene rs7101916snp in the preparation and detection of hepatitis C susceptibility products

A genotype and gene technology, which is applied in the application field of preparation and detection of hepatitis C susceptibility products

Active Publication Date: 2019-11-08
JIANGSU PROVINCE HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the relationship between the polymorphism of the RelA gene rs7101916 single nucleotide site and the susceptibility to hepatitis C infection at home and abroad

Method used

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  • Application of rela gene rs7101916snp in the preparation and detection of hepatitis C susceptibility products
  • Application of rela gene rs7101916snp in the preparation and detection of hepatitis C susceptibility products
  • Application of rela gene rs7101916snp in the preparation and detection of hepatitis C susceptibility products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of substances for detecting the single nucleotide polymorphism or genotype of the RelA gene rs7101916 in the human genome

[0062] 1. Genomic DNA extraction

[0063] (1) Collect 5ml of peripheral venous blood from patients in EDTA anticoagulant tubes, centrifuge at 4000rpm for 10min, separate serum, white blood cells and red blood cells, number them one by one after aliquoting, and freeze them at -80°C for later use.

[0064] (2) Genomic DNA was extracted by the phenol-chloroform method: take 3 times the volume of cell lysate and add it to blood cells after centrifugation, shake and mix well, lyse at room temperature for 5 minutes, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0065] (3) Observe the color of the precipitate. If the red color is darker, continue to add cell lysate, break and remove red blood cells until the centrifuged precipitate is white or light pink.

[0066] (4) Take 1ml of genomic DNA extract and 8μl of...

Embodiment 2

[0110] Example 2 Application of RelA gene rs7101916 single nucleotide polymorphism or genotype

[0111] 1. Detection steps

[0112] The substance obtained in Example 1 for detecting the single nucleotide polymorphism or genotype of RelA gene rs7101916 in the human genome (ie the system solution prepared according to the system in Table 2) was divided into 8 sterile PCR tubes. Adjust the volume of the discharge gun to 5 μl, take the system solution and add it to a 384-well plate, and finally add 1 μl / well of the DNA sample. Set 2 blank controls (sterile double distilled water) and 2 known positive controls on each plate to control reagents, system contamination and test results.

[0113] (1) Seal the 384-well plate containing the sample with a special Taqman PCR sealing film, and centrifuge for 1 min. Install the 7900HT fluorescent quantitative PCR instrument and set the PCR reaction conditions: pre-denaturation at 50°C for 5 minutes, initial denaturation at 95°C for 10 minut...

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Abstract

The invention discloses applications of human genome NF-kappaB pathway RelA gene rs7101916 single nucleotide polymorphism (SNP) or genotype in preparation of products used for detecting or screening hepatitis C infectibility or in preparation of hepatitis C-related single nucleotide polymorphism products. In practical applications, rs7101916 single nucleotide polymorphism (i.e. allelic genes) or genotype substances are combined with other substances (such as other hepatitis C-related single nucleotide polymorphism or genotype substances) to prepare the products used for screening patients withhepatitis C infectibility.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of the RelA gene rs7101916 single nucleotide polymorphism (single nucleotide polymorphism, SNP) of the NF-κB pathway in the human genome in the preparation and detection of hepatitis C susceptibility products. Background technique [0002] SNP refers to a change in nucleic acid sequence caused by a single nucleotide mutation. There are two alleles at a SNP locus. In the human genome, there is one SNP in every 100-300 nucleotides on average. SNPs are the most common type of genetic variation in humans. SNPs are distributed in both coding and non-coding regions of the human genome, and most SNPs exist in non-coding regions. SNPs in the coding region have non-synonymous mutations and synonymous mutations. Non-synonymous SNPs can change the amino acid sequence of proteins, while synonymous mutations do not affect protein coding. SNPs in non-coding regions may affect gene ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
Inventor 岳明李军喻荣彬刘源韩亚萍金柯田亭张云
Owner JIANGSU PROVINCE HOSPITAL
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