Method for extracting polysaccharides from passion fruit peel

A technology of passion fruit and pericarp, which is applied in the field of extracting polysaccharides, can solve the problems that have not been reported, and achieve the effects of retaining physiological activity, easy control, and high extraction rate

Inactive Publication Date: 2018-01-12

HEZHOU UNIV

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AI-Extracted Technical Summary

Problems solved by technology

But so far, the method for efficiently extracting passio...

Abstract

The invention discloses a method for extracting polysaccharides from passion fruit peel. The method comprises the following steps: (1) soaking the passion fruit peel into hot water, and carrying out ultrasonic extraction and centrifugation; and (2) filtering an extracting solution obtained in the step (1) by virtue of an ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate, and carrying out reduced pressure concentration, so as to obtain passion fruit peel polysaccharides. The method has the advantages that the pollution, the solvent residue and the phase change are avoided, the extraction rate is high, the product quality is good, the operation is simple, and the control is convenient, and the like.

Technology Topic

UltrafiltrationSolvent +8

Examples

Experimental program(4)

Example Embodiment

[0017] Example 1:

[0018] A method for extracting polysaccharides from passion fruit peel of the present invention includes the following steps:

[0019] (1) Raw material processing: Wash the passion fruit peel and slice it in a drying cabinet at about 55°C, then place it in a pulverizer to pulverize, and filter through an 80-mesh standard sieve to obtain passion fruit peel powder;

[0020] (2) Ultrasonic extraction: The passion fruit peel powder obtained in step (1) is immersed in hot water, ultrasonically extracted, and the material-to-liquid ratio (that is, the ratio of passion fruit peel powder to hot water) is 1g:35mL, and the extraction temperature is 55℃, ultrasonic leaching power is 150W, leaching time is 24min, after leaching, 6000rpm centrifugal filtration for 15min, immersing the filter residue in hot water, ultrasonic extraction and filtering according to the above extraction process; repeat the filter residue immersion-ultrasonic- The filtration process is 3 times, and the filtrate is combined to obtain the extract;

[0021] (3) Ultrafiltration membrane retention and concentration: the extract obtained in step (2) is filtered with an ultrafiltration membrane (with a molecular weight cut-off of 10000D), when the mass concentration of polysaccharides in the filtrate reaches 10%, add anhydrous to the filtrate The volume fraction of ethanol to ethanol is 70%, stirred and allowed to stand, and the resulting mixture is concentrated under reduced pressure at 4° C. and a vacuum degree of 0.1 MPa to obtain a concentrate.

[0022] (4) Freeze drying: the concentrate obtained in step (3) is washed with absolute ethanol and then freeze dried, dried and then pulverized to obtain passion fruit peel polysaccharide.

[0023] After testing, the yield of passion fruit peel polysaccharide obtained in this example was 8.02%.

Example Embodiment

[0024] Example 2:

[0025] A method for extracting polysaccharides from passion fruit peel of the present invention includes the following steps:

[0026] (1) Raw material processing: After the passion fruit peel is washed, the slices are dried in a drying box at about 55°C, and then placed in a grinder to pulverize, and then filtered through a standard sieve of 80 mesh to obtain passion fruit peel powder;

[0027] (2) Ultrasonic extraction: The passion fruit peel powder obtained in step (1) is immersed in hot water, ultrasonically extracted, the material-to-liquid ratio (that is, the ratio of passion fruit peel powder to hot water) is 1g:4mL, and the extraction temperature is 60℃, ultrasonic leaching using power 200W, leaching time 32min, after leaching, 6000rpm centrifugal filtration for 15min, immersing the filter residue in hot water, ultrasonic extraction and filtration according to the above extraction process; repeat the filter residue immersion-ultrasonic-filtration Process 3 times, combine the filtrate to obtain the extract;

[0028] (3) Ultrafiltration membrane retention and concentration: the extract obtained in step (2) is filtered with an ultrafiltration membrane (with a molecular weight cut-off of 10000D), when the mass concentration of polysaccharides in the filtrate reaches 10%, add anhydrous to the filtrate The volume fraction of ethanol to ethanol is 70%, stirred and allowed to stand, and the resulting mixture is concentrated under reduced pressure at 4° C. and a vacuum degree of 0.1 MPa to obtain a concentrate.

[0029] (4) Freeze drying: the precipitate in step (3) is washed with absolute ethanol and then freeze dried, dried and then pulverized to obtain passion fruit peel polysaccharide.

[0030] After testing, the yield of passion fruit peel polysaccharide obtained in this example was 9.61%.

Example Embodiment

[0031] Example 3:

[0032] (1) Raw material processing: After the passion fruit peel is washed, the slices are dried in a drying box at about 55°C, and then placed in a grinder to pulverize, and then filtered through a standard sieve of 80 mesh to obtain passion fruit peel powder;

[0033] (2) Ultrasonic extraction: The passion fruit peel powder obtained in step (1) is immersed in hot water, ultrasonically extracted, and the material-to-liquid ratio (that is, the ratio of passion fruit peel powder to hot water) is 1g:45mL, and the extraction temperature is 65°C, ultrasonic leaching using power 250W, leaching time 40min, after leaching, 6000rpm centrifugal filtration for 15min, immersing the filter residue in hot water, ultrasonic extraction and filtration according to the above extraction process; repeat the filter residue immersion-ultrasonic-filtration Process 3 times, combine the filtrate to obtain the extract;

[0034] (3) Ultrafiltration membrane retention and concentration: the extract obtained in step (2) is filtered with an ultrafiltration membrane (with a molecular weight cut-off of 10000D), when the mass concentration of polysaccharides in the filtrate reaches 10%, add anhydrous to the filtrate The volume fraction of ethanol to ethanol is 70%, stirred and allowed to stand, and the resulting mixture is concentrated under reduced pressure at 4° C. and a vacuum degree of 0.1 MPa to obtain a concentrate.

[0035] (4) Freeze drying: the precipitate in step (3) is washed with absolute ethanol and then freeze dried, dried and then pulverized to obtain passion fruit peel polysaccharide.

[0036] After testing, the yield of the passion fruit peel polysaccharide obtained in this example was 11.21%.

PUM

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Classification and recommendation of technical efficacy words

High extraction rate
easy to operate

Method for extracting polysaccharides from passion fruit peel

A technology of passion fruit and pericarp, which is applied in the field of extracting polysaccharides, can solve the problems that have not been reported, and achieve the effects of retaining physiological activity, easy control, and high extraction rate

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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