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Preparation method of a homoarginine mixed peptide and its application in the treatment of breast cancer

A technology of high arginine and mixed peptides, which is applied in the field of preparation of high arginine mixed peptides to achieve the effect of reducing absorption inhibition, delaying deterioration, and expanding production potential

Active Publication Date: 2021-02-05
陈栋梁 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method of a homoarginine mixed peptide and its application in the treatment of breast cancer
  • Preparation method of a homoarginine mixed peptide and its application in the treatment of breast cancer
  • Preparation method of a homoarginine mixed peptide and its application in the treatment of breast cancer

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Embodiment 1

[0034] The preparation method of homoarginine mixed peptide of the present invention comprises the following steps:

[0035] 1. Mix the degreased and crushed walnut meal with egg white protein at a mass ratio of 4:1, mix the protein meal mixture with water at a mass-volume ratio of 1:8, stir at room temperature for 2 hours, and then place it in an ultra-high pressure host system , the treatment pressure is 400MPa, and the holding time is 20min to obtain a suspension pretreated by ultra-high pressure; control the temperature of the suspension to 50°C, adjust the pH to 9, add alkaline protease with a mass of 3.5% of the suspension and stir evenly , at the same time, ultrasonic-microwave assisted enzymolysis, ultrasonic power 300W, ultrasonic time 12min, microwave power 400W, microwave time 8min, after 2h of enzymolysis, adjust the pH to 7, add papain with a mass of 3.5% of the suspension and stir evenly, while ultrasonic -Microwave-assisted enzymatic hydrolysis, ultrasonic power...

Embodiment 2

[0048] The preparation method of the mixed peptide of the present invention comprises the following steps:

[0049] 1. Mix the degreased and crushed walnut meal with egg white protein at a mass ratio of 3:1, mix the protein meal mixture with water at a mass-volume ratio of 1:6, stir at room temperature for 1.5 hours, and then place it in the ultra-high pressure host system In the middle, the treatment pressure is 300MPa, and the holding time is 15min to obtain a suspension pretreated by ultra-high pressure; control the temperature of the suspension to 50°C, adjust the pH to 9, add 3% alkaline protease of the suspension mass and stir Uniform, simultaneous ultrasonic-microwave assisted enzymolysis, ultrasonic power 200W, ultrasonic time 15min, microwave power 300W, microwave time 10min, after 1.5h of enzymolysis, adjust the pH to 7, add papain with 3% mass of the suspension and stir evenly, Simultaneously, ultrasonic-microwave assisted enzymolysis, ultrasonic power 200W, ultraso...

Embodiment 3

[0054] The preparation method of the mixed peptide of the present invention comprises the following steps:

[0055]1. Mix the degreased and crushed walnut meal particles with egg white protein at a mass ratio of 5:1, mix the protein meal mixture with water at a mass-volume ratio of 1:10, stir at room temperature for 2.5 hours, and place it in an ultra-high pressure host In the system, the processing pressure is 500MPa, and the pressure holding time is 25min to obtain a suspension pretreated by ultra-high pressure; the temperature of the suspension is controlled to 50°C, the pH is adjusted to 9, and 4% of the mass of the suspension is added to alkaline protease and Stir evenly, and at the same time, ultrasonic-microwave assisted enzymolysis, ultrasonic power 400W, ultrasonic time 20min, microwave power 500W, microwave time 5min, after 2.5h of enzymolysis, adjust the pH to 7, add papain with 4% mass of the suspension and stir well At the same time, ultrasonic-microwave assisted ...

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Abstract

The invention relates to a mixed peptide with high arginine content and its application in the treatment of breast cancer. The preparation method comprises the following steps: pretreating the walnut meal and egg white protein suspension by ultra-high pressure, and then using alkaline protease and papain to enzymolyze the pretreated product step by step under the assistance of ultrasonic waves and microwaves; enzymatic hydrolysis The supernatant liquid after liquid filtration was separated by reversed-phase high performance liquid chromatography to separate the target mixed peptide; the mixed peptide was used as template molecule, acrylic acid and methacrylic acid were functional monomers, triethylene glycol dimethacrylate was The coupling agent, isopropylthioxanthone as the initiator, UV-induced polymerization to form a surface molecularly imprinted membrane, which is used to separate and enrich the target mixed peptides in the enzymatic hydrolysis solution. The content of arginine in the mixed peptide is more than 18%; it is found by the MTT method that the homo-arginine mixed peptide can effectively inhibit the proliferation of human breast cancer MCF‑7 cells. This law helps to reduce product cost and accelerate the realization of industrialized production.

Description

technical field [0001] The present invention relates to a method for preparing a high-arginine mixed peptide and its application in the treatment of breast cancer, in particular to a method for preparing arginine-rich mixed peptide with walnut meal and egg white protein as raw materials and its application in Applications in the treatment of breast cancer. Background technique [0002] Cancer is a general term for malignant tumors, characterized by the loss of normal regulatory control of body cells, excessive proliferation, and different degrees of differentiation disorders, and often invades adjacent tissues or metastasizes to distant sites. It seriously threatens the health of all human beings and ranks first among the causes of abnormal death. According to the "Global Cancer Report" released by the World Health Organization (WHO) in 2014, the number of new cancer cases in China ranked first in the world in 2012. In 2015, the "Three-Year Action Plan for Cancer Preventio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/18A23L33/18A61K38/01A61K35/00
Inventor 陈栋梁张阳刘立坚何立蓉
Owner 陈栋梁
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