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Application of GNL2 protein to preparation of liver cancer postoperative prognosis evaluation kit, liver cancer postoperative prognosis evaluation kit and method

A prognostic evaluation and kit technology, applied in the biological field, can solve the problems of lack of specific molecular targets and unclear molecular mechanisms in treatment

Active Publication Date: 2018-01-12
厦门柏恒生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Liver cancer is one of the most threatening tumors to human health. So far, the molecular mechanism of its occurrence is still unclear, and its treatment lacks specific molecular targets. As a very important tumor oncogene, GNL2 is currently unknown. Literature reports that GNL2 protein is related to judging the prognosis or metastasis of liver cancer

Method used

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  • Application of GNL2 protein to preparation of liver cancer postoperative prognosis evaluation kit, liver cancer postoperative prognosis evaluation kit and method
  • Application of GNL2 protein to preparation of liver cancer postoperative prognosis evaluation kit, liver cancer postoperative prognosis evaluation kit and method
  • Application of GNL2 protein to preparation of liver cancer postoperative prognosis evaluation kit, liver cancer postoperative prognosis evaluation kit and method

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Embodiment 1

[0042] A kit for postoperative prognosis assessment of liver cancer, the kit includes: human GNL2 monoclonal antibody or GNL2 polyclonal antibody, and immunohistochemical reagents for detecting the relative expression of GNL2 protein in liver cancer tissue. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0043] The human GNL2 polyclonal antibody of the present invention is obtained by immunizing rabbits with the GNL2 protein whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0044] Immunohistochemical experiment reagent of the present invention comprises: Xylene, ethanol, 3%H 2 o 2 (aqueous solution), 3% BSA blocking solution (prepared in PBS), DAB chromogenic reagent, hematoxylin, horseradish peroxidase (for labeling secondary antibody), PBS (pH7.4)...

Embodiment 2

[0046] The application of GNL2 protein in the preparation of the postoperative prognosis assessment kit for liver cancer, the application is to use the kit of the present invention for postoperative prognosis assessment of liver cancer, the method is as follows:

[0047] (1) Take pathological specimens of patients with liver cancer, and use human GNL2 monoclonal antibody or human GNL2 polyclonal antibody, as well as immunohistochemical experimental reagents, for immunohistochemical staining;

[0048] Among them, the pathological specimens come from the biopsy tissue of patients with liver cancer or the pathological materials taken during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0049] (a) Paraffin sections of liver cancer tissues were prepared, and the oven was set at 60°C overnight.

[0050] (b) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[...

Embodiment 3

[0069] A postoperative tumor sample of liver cancer was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the GNL2 immunohistochemical score of the cancer tissue was 0.216. After postoperative follow-up, it was found that the patient had recurrence and metastasis of liver cancer in the 8th month after the operation, and died in the 19th month after the operation.

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Abstract

The invention provides application of a GNL2 protein to preparation of a liver cancer postoperative prognosis evaluation kit, the liver cancer postoperative prognosis evaluation kit and a method. Therelative expression quantity of the GNL2 protein in liver cancer tissues is detected by an immunohistochemical method, so that the risk that liver cancer recurrence and metastasis occurs in patients suffering from liver cancer is judged. The invention has the following beneficial effects: the invention provides the application of the GNL2 protein to preparation of the liver cancer postoperative prognosis evaluation kit, and indicates that the protein can be used for preparing a protein molecular maker for judging prognosis of the patients suffering from the liver cancer and has important guiding significance in prognosis monitoring and sequential treatment of the patients suffering from the liver cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of GNL2 protein in the preparation of a liver cancer prognosis evaluation kit, the liver cancer prognosis evaluation kit and a method. Background technique [0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. The tumor markers of most patients with secondary liver cancer are within the normal range, but a small number of liver metastases from the stomach, esophagus, pancreas and ovary may have elevated AFP. Symptomatic patients are often accompanied by elevated ALP and GGT. Elevated carcinoembryonic antigen CEA is helpful for the diagnosis of liver metastases, and the positive rate of CEA in colorectal cancer liver metastases is as high as 60% to 70%. A lesion with a diameter of 1 cm can be found on selective hepatic angiography. Selective peritoneal or hepatic arteriography often shows...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
Inventor 林尧王清水
Owner 厦门柏恒生物科技有限公司