A kind of Cordyceps sinensis liquid culture medium and the method for cultivating Cordyceps sinensis using culture medium

A liquid culture medium and the technology of Cordyceps sinensis, which is applied in the field of microorganisms, can solve the problems of mycelium property differences, poor quality of Cordyceps sinensis, and low nutrient content, and achieve the effects of increased strain utilization, good strain attachment performance, and increased corporate profits

Active Publication Date: 2020-11-03
邸超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many individuals and units in China have researched and disclosed Cordyceps sinensis culture medium and fermentation production methods. However, these technologies mostly use common culture medium for microbial fermentation, and basically use eggs, milk, and potatoes as fermentation food sources, although they are all based on microbiological fermentation. The principle enables the strains to grow, but the different food sources will inevitably lead to differences in the properties of the mycelium, and the cost of the culture medium is relatively high. The chemical composition and active substances of the fermented products of Cordyceps sinensis produced are different from those of natural Cordyceps sinensis. Cordyceps sinensis cultivated in the prior art generally has the problems of poor quality and low nutrient content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of Cordyceps sinensis liquid culture medium, it is prepared according to the following process::

[0034] 1) Mix sawdust and straw powder, then soak in NaOH solution with a concentration of 2mol / L for 5 hours, remove the filamentous fibers, put them in clean water, stir and rinse them twice at high speed, and then dry them at 60°C to obtain fibrous fibers. substance;

[0035] 2) Put the distiller's grains into the reactor, then add twice the weight of hydrochloric acid with a concentration of 5 mol / L, stir and hydrolyze at 300 rpm for 2 hours, then add ammonia water, adjust the pH of the solution to 6.8, and obtain the distiller's grains hydrolyzate;

[0036] 3) Peel the potatoes, cut them into pieces, then add them to twice the weight of distilled water, boil for 30 minutes, cool naturally, then filter with gauze, collect the filtrate, and obtain the potato extract;

[0037] 4) Take 50g of the fibrous substance obtained in step 1), 50g of distiller's grain hydr...

Embodiment 2

[0039] A kind of Cordyceps sinensis liquid culture medium, it is prepared according to the following process::

[0040]1) Mix sawdust and straw powder, then soak in NaOH solution with a concentration of 3mol / L for 3 hours, remove the filamentous fibers, put them in clean water, stir and rinse them at high speed for 3 times, and then dry them at 70°C to obtain fibrous fibers. substance;

[0041] 2) Put the distiller's grains into the reactor, then add twice the weight of hydrochloric acid with a concentration of 5 mol / L, stir and hydrolyze at 300 rpm for 2 hours, then add ammonia water, adjust the pH of the solution to 6.6, and obtain the distiller's grains hydrolyzate;

[0042] 3) Peel the potatoes, cut them into pieces, then add them to twice the weight of distilled water, boil for 30 minutes, cool naturally, then filter with gauze, collect the filtrate, and obtain the potato extract;

[0043] 4) Take 60g of the fibrous substance obtained in step 1), 60g of distiller's grain...

Embodiment 3

[0045] Adopt the liquid culture medium of embodiment 1 to cultivate Cordyceps sinensis, comprise the steps:

[0046] The first step, the slant cultivation of the first-class mother species of Cordyceps sinensis:

[0047] (1) Medium formula: 200 grams of corn, 200 grams of wheat, 4 grams of potassium dihydrogen phosphate, 20 grams of glucose, 1 gram of magnesium sulfate, 20 grams of peptone, 1 gram of streptomycin, 1 gram of penicillin, 20 grams of agar, Vitamin B1 0.01g, water 1000ml;

[0048] (2) Preparation method: Add 1000 ml of water to the cleaned corn and wheat, boil on high heat and boil on low heat for 40 minutes, filter off the residue of corn and wheat with gauze, take the juice and add 4 grams of potassium dihydrogen phosphate, glucose 20 grams, 1 gram of magnesium sulfate, 20 grams of peptone, 1 gram of streptomycin, 1 gram of penicillin, 20 grams of agar, vitamin B 1 0.01g and stir evenly, continue to heat until the agar is completely melted, and quickly divide...

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PUM

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Abstract

The invention belongs to the field of microorganism technology, and discloses a cordyceps sinensis fluid medium. The cordyceps sinensis fluid medium is prepared from 50 to 60g of a fibrous material, 50 to 60g of a vinasse hydrolysate, 70 to 80g of potato extraction solution, 30 to 40g of glucose, 2g of magnesium sulfate, 1g of streptomycin, 1g of penicillin, and 0.01g of vitamin B1. A preparationmethod comprises following steps: the above ingredients are delivered into a container, an obtained mixture is diluted with water to 1L, the pH value is adjusted to 6.5 to 6.8, sterilization in an autoclave is carried out, wherein the pressure is maintained for 15min when the temperature is increased to 121 DEG C, an obtained product is taken out from the autoclave, and is cooled to room temperature. The invention also discloses a method used for culturing cordyceps sinensis with the cordyceps sinensis fluid medium. The cordyceps sinensis fluid medium is reasonable in composition, and low in cost; and the quality of cultured products is improved greatly.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a liquid culture medium of Cordyceps sinensis and a method for cultivating Cordyceps sinensis using the medium. Background technique [0002] Cordyceps sinensis is a complex of fruiting bodies (fruiting bodies) and larvae grown by Cordyceps sinensis infected and parasitized on the larvae of bat moths. It is an edible fungus and is a traditional precious Chinese medicine in my country. It has various effects such as regulating immune system function, anti-tumor, and anti-fatigue. [0003] Bat moth larvae are infected by Cordyceps sinensis fungus (Cordyceps sinensis), the mycelium fills the whole body tissue and forms a hard pseudosclerotia, which remains unchanged for several months in the low-temperature and dry soil in winter (winter worm). When the temperature and humidity are suitable in summer, rod-shaped fruiting bodies (sperms) grow from the sclerotia and are expos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/00C12R1/645
Inventor 邸超
Owner 邸超
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