Molecular marker affecting waist traits of Duroc pigs and application

A molecular marker and trait technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of lack of systematic and detailed research on waist circumference phenotype selection and measurement, economic losses of pig breeding enterprises, breeding pigs. Reduce the utilization rate and other problems, to achieve the effect of improving the body type selection process, increasing sales profits, and improving core competitiveness

Active Publication Date: 2018-01-16
WENS FOOD GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large belly circumference, small waist circumference, and uneven abdominal line, commonly known as big belly and big belly, this kind of pig has a lot of fat, which leads to a large loss in trimming of pigs, which affects the profit of the final sale of breeding pigs
In addition, due to the large belly of the breeding pigs, it is not uncommon for commercial pig farms to be unwilling to introduce breeds, which reduces the utilization rate of such breeding pigs, which has brought huge economic losses to pig farming enterprises
Because the choice of body shape needs to take into account a lot, it is difficult to take into account the waist circumference while selecting other traits, which brings difficulties to the improvement of waist circumference
Phenotypic selection and measurement of waist circumference has not been systematically and exhaustively studied

Method used

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  • Molecular marker affecting waist traits of Duroc pigs and application
  • Molecular marker affecting waist traits of Duroc pigs and application
  • Molecular marker affecting waist traits of Duroc pigs and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Experimental animals

[0033] The experimental pig group used in this example is 627 purebred American Durocs of Guangdong Wenshi Breeding Pig Technology Co., Ltd., which is the core group of the breeding pig branch company, and the group pedigree records are detailed.

[0034] In this experiment, Chinese and American Duroc pigs were selected from this resource group. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0035] Duroc pigs have a small number of litters, with an average of only 9 to 10 pigs in a large group, but they grow fast, have a high feed conversion rate, a high carcass lean meat rate, a high intramuscular fat content, and strong stress resistance. Domestic commercial pig production has established a relatively mature hybrid supporting system. Among them, Du Dachang's hybrid combination continues to maintain an absolute dominant position in my ...

Embodiment 2

[0049] Example 2 Target DNA sequence amplification and sequencing

[0050] (1) Primer design

[0051] The DNA sequence of SEQ ID NO.1 on pig chromosome 4 was downloaded from Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers. The DNA sequences of the designed primers are as follows:

[0052] Primer C-F: 5'-GGGTGAGGGTGGGATTAG-3';

[0053] Primer C-R: 5'-TTGCGTTAGTGATTGTCCTG-3';

[0054] (2) PCR amplification

[0055] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMixwithLoading Dye, and 0.3 μL of primers C-F and C-R into the 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 54.3°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0056] (3) DNA sequence determination

[0057] Finally, the PCR-amplified products were sequenced, and the seq...

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Abstract

The invention belongs to the technical field of molecular marker-assisted selection and animal genetic breeding and particularly relates to a molecular marker affecting waist traits of Duroc pigs andan application. An SNP locus of the molecular marker affecting the waist traits of the Duroc pigs corresponds to T>C mutation on the 3400805th nucleotide locus of chromosome 4 in a reference sequencein international swine genome version 10.2. The invention further provides a primer pair for identifying the molecular marker affecting the waist traits of the Duroc pigs. By means of the molecular marker and the primer pair, an efficient and accurate molecular marker-assisted breeding technology can be established, after the technology is applied to genetic improvement of the waist traits of theDuroc pigs, waist of offspring pigs is increased, pork yield is increased, profit of enterprises is increased, and core competence is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular marker-assisted selection and animal genetic breeding, and specifically relates to a molecular marker that affects the waistline traits of Duroc pigs and its application. Background technique [0002] In the market environment in which meat pigs are mainly evaluated as live pigs in China, the selection of body shape and phenotype is particularly important. Foreign breeding pig markets seldom judge their value based on their body shape. However, they still make a comprehensive evaluation and selection of the pig's forelimbs, hindlimbs, movement status, posture, back, belly line and other body appearance. In most of the pig markets in China, the belly line (abdominal circumference, waist circumference) determines the value of the meat pig and directly affects the sales profit of the end product. Due to the large abdominal circumference, small waist circumference, and uneven abdominal line, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 杨明吴珍芳杨杰丁荣荣陈赞谋王青来蔡更元曾海玉
Owner WENS FOOD GRP CO LTD
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