Feed additive capable of improving piglet immunity
A feed additive and immunity technology, applied in the field of piglet feed additives, can solve problems that have not yet been seen, achieve the effects of no toxic side effects, improve piglet immunity, and significant curative effect
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Embodiment 1
[0040] Embodiment 1, the extraction of bletilla striata polysaccharide
[0041] Bletilla striata tuber dried products were crushed, sieved, dried, added 20 times the volume of distilled water, refluxed at 60°C for 4 hours, repeated extraction once, and combined the two extracts. Centrifuge at 5000rpm for 10min, take the supernatant and filter it with suction, concentrate under reduced pressure at 70°C to a smaller volume, add 3 times the volume of absolute ethanol to precipitate, dissolve the precipitate in the minimum volume of water, and deproteinize it by Sevag method until it is detected by Coomassie brilliant blue G-250 method No protein was detected. The obtained solution was dialyzed with distilled water for 5 days, the relative molecular mass cut-off of the dialysis bag was 8000-10000, and vacuum freeze-dried.
Embodiment 2
[0042] Embodiment 2, the preparation of feed additive
[0043] Take 5 parts by weight of oxymatrine and 5 parts by weight of Bletilla striata polysaccharide prepared in Example 1, and mix them to make feed additive S1 with a weight ratio of oxymatrine and Bletilla striata polysaccharide in a 1:1 weight ratio.
[0044] Take 4 parts by weight of oxymatrine and 6 parts by weight of Bletilla striata polysaccharide prepared in Example 1, and mix them to make feed additive S2 with a weight ratio of oxymatrine and Bletilla striata polysaccharide of 2:3.
[0045] Take 1 part by weight of oxymatrine and 9 parts by weight of Bletilla striata polysaccharide prepared in Example 1, and mix them to prepare feed additive S3 with a weight ratio of oxymatrine and Bletilla striata polysaccharide in a weight ratio of 1:9.
Embodiment 3
[0046] Embodiment 3, the application of feed additive of the present invention in improving piglet immunity
[0047] 1. Experimental animals and grouping treatment
[0048] Select 20-day-old piglets with clinical health and similar body weight, take blood samples to detect the maternal antibody level of swine fever, select 120 piglets with similar antibody levels, and divide them into 6 groups at random, i.e. Chinese medicine composition groups 1-3 of the present invention (Example 1). 2 Prepared feed additives S1-S3), oxymatrine group, bletilla striata polysaccharide group and control group, 20 piglets in each group. At the age of 21 days, the rabbits were immunized with attenuated swine fever rabbit vaccine. At the age of 27-31 days, add the above-mentioned feed additives according to 1% of the total weight of the feed in the diets of the S1-S3 Chinese medicine composition group, oxymatrine group, and bletilla striata polysaccharide group (the total amount of active ingredi...
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