Unlock instant, AI-driven research and patent intelligence for your innovation.

Comprehensive extraction method of biological salt and betacyanin from suaeda salsa

A technology of betalain and Suaeda salsa, applied in chemical instruments and methods, food science, azo dyes, etc., can solve the problems of low yield, low product purity, and no reports on simultaneous extraction processes or methods. , to achieve the effect of improving purity, short processing time and preventing degradation

Inactive Publication Date: 2018-01-19
WEIFANG YOURONG IND
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies have confirmed that Suaeda salsa is rich in betalain. In the prior art, there are reports of extracting betalain or biological salt using Suaeda salsa as raw material, but they are all based on the extraction of a certain component. method, and the resulting product has low purity and low yield, and the technique or method for simultaneous extraction of betalain and biological salt has not been reported. Therefore, for the above problems, it is necessary to establish a comprehensive extraction method for biological salt and betaine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] A method for comprehensively extracting Suaeda salsa biological salt and betaine, comprising the following steps:

[0028] (1) raw material pretreatment: the freshly picked Suaeda salina stems and leaves are washed and dried, and an extraction solvent (40% ethanol and 0.1M citric acid with a volume concentration of 1:5) is added according to the ratio of solid to liquid Mixed according to the ratio of 1:0.5), flash extraction (extraction time is 1.5min, extraction temperature is 25°C, extraction voltage is 180v), filtered, and supernatant and precipitate are collected respectively;

[0029] (2) Ultrasound-assisted enzymolysis treatment: get the precipitate that step (1) collects, add the compound enzyme (by pectinase, cellulase, neutral protease and α-glucosidase according to 1:2:1:1 ratio), mix well, adjust pH, after ultrasonic treatment (ultrasonic power 220W, ultrasonic time 5min) for enzymolysis reaction (enzymolysis condition is pH3.5, temperature 25 °C, the enzym...

example 2

[0035] A method for comprehensively extracting Suaeda salsa biological salt and betaine, comprising the following steps:

[0036] (1) raw material pretreatment: the freshly picked Suaeda salsa stems and leaves are washed and dried, and an extraction solvent (45% ethanol and 0.3M citric acid with a volume concentration of 1:8) is added according to the ratio of solid to liquid Mixed according to the ratio of 1:0.5), flash extraction (extraction time is 2min, extraction temperature is 28°C, extraction voltage is 185v), filtered, and supernatant and precipitate are collected respectively;

[0037] (2) Ultrasound-assisted enzymolysis treatment: get the precipitate that step (1) collects, add the compound enzyme (by pectinase, cellulase, neutral protease and α-glucosidase according to 2:3:2:1.5 ratio), mix well, adjust pH, after ultrasonic treatment (ultrasonic power 260W, ultrasonic time 8min) for enzymolysis reaction (enzymolysis condition is pH3.5, temperature 28 °C, the enzyma...

example 3

[0043] A method for comprehensively extracting Suaeda salsa biological salt and betaine, comprising the following steps:

[0044] (1) raw material pretreatment: the freshly picked Suaeda salsa stems and leaves are washed and dried, and an extraction solvent (75% ethanol and 0.45M citric acid with a volume concentration of 1:10) is added according to the ratio of solid to liquid Mixed according to the ratio of 1:0.5), flash extraction (extraction time is 4min, extraction temperature is 28.5°C, extraction voltage is 202v), filtered, and supernatant and precipitate are collected respectively;

[0045] (2) Ultrasound-assisted enzymolysis treatment: get the precipitate that step (1) collects, add the compound enzyme (by pectinase, cellulase, neutral protease and α-glucosidase according to 4.5:6:5:2.7 ratio), mix well, adjust pH, after ultrasonic treatment (ultrasonic power is 420W, ultrasonic time is 12min) for enzymolysis reaction (enzymolysis condition is pH6.2, temperature 36 ℃...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of extraction of betacyanin, in particular to a comprehensive extraction method of biological salt and betacyanin from suaeda salsa. The method comprises the following steps of performing pretreatment, performing ultrasonic treatment for assisting in enzymolysis treatment, performing super critical extraction, performing subcritical extraction, performing purification and performing drying. Through the utilization of the extraction method, the extraction rate and the purity of suaeda salsa betacyanin products can be greatly increased, the yield of the biological salt is high, and the time for decoloring and removing heavy metals for the biological salt is short.

Description

technical field [0001] The invention relates to the technical field of betalain extraction, in particular to a method for comprehensively extracting Suaeda salsa biological salt and betalain. Background technique [0002] Betalain is a kind of natural plant pigment that widely exists in plants. It is composed of red betalain and betaflavin. The main component of betalain is betalain, which accounts for 75-95% of red pigment. There are isobetaine, former betaine and so on. Betalain, its anticancer, antiviral and antioxidative biological activities make betalain widely concerned in the field of health care products and food additives. [0003] Suaeda salsa is a typical true halophyte, which is widely distributed on saline land such as coastal tidal flats. It is a natural resource with great development potential and has great economic, ecological and social benefits. Current studies have confirmed that Suaeda salsa is rich in betalain. In the prior art, there are reports of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L27/40C09B61/00C09B67/54
Inventor 王胜
Owner WEIFANG YOURONG IND