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Method for extracting and purifying polyphyllin I from rhizoma paridis

A technology for purifying Cholesterol and saponins, applied in organic chemistry, steroids, etc., can solve the problems of low yield, difficult to achieve large-scale production, low purity, etc., and achieve high purity, high production efficiency, and fast extraction speed. Effect

Active Publication Date: 2018-01-23
广西产学研信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention discloses a method for extracting and purifying saponin Ⅰ from Chonglou, in order to solve the problems of low yield, low purity and difficulty in realizing large-scale production in existing extraction methods

Method used

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  • Method for extracting and purifying polyphyllin I from rhizoma paridis

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Embodiment 1

[0023] A method for extracting and purifying saponin Ⅰ from Chongjiao, comprising the following steps:

[0024] S1: Grind Chonglou into a coarse powder, put it in a closed container, and ferment the inoculated strain at 55°C for 36 hours. Sterilize, add 7 times of 80% ethanol, microwave heating and reflux extraction twice, each extraction 10min, and control the reflux heating temperature at 90°C by controlling the amount of microwave radiation, filter after extraction, and combine extraction liquid;

[0025] S2: Use activated carbon to decolorize the extract in step S1, then use acetone to degrease, place it in a rotary evaporator at 60°C, depressurize it to 0.01MPa, and concentrate it until the content of pachyphyllin saponin is 10%; add to the concentrated solution Add 75% ethanol solution, centrifuge continuously for 3 times in a centrifuge at 3000r / min, and combine the supernatant;

[0026] S3: Pass the supernatant in step S2 through the D101 macroporous adsorption resin...

Embodiment 2

[0029] A method for extracting and purifying saponin Ⅰ from Chongjiao, comprising the following steps:

[0030] S1: Grind Chonglou into coarse powder, put it in a closed container, and ferment the inoculated strain at 56°C for 42 hours. The strain is a composition of yeast, actinomycetes, and bacillus. Distilled water was washed 3 times, the water was filtered and sterilized, 8 times of 90% ethanol was added, microwave heating and reflux extraction was performed twice, each extraction was 12min, and the temperature of reflux heating was controlled by controlling the amount of microwave irradiation at the same time. 93°C, filter after the extraction, and combine the extracts;

[0031] S2: Use activated carbon to decolorize the extract in step S1, then use acetone to degrease, place it in a rotary evaporator at 32°C, depressurize it to 0.015MPa, and concentrate it until the content of papaya saponin is 12%; add to the concentrated solution Add 80% ethanol solution, centrifuge c...

Embodiment 3

[0035] A method for extracting and purifying saponin Ⅰ from Chongjiao, comprising the following steps:

[0036] S1: Grind Chonglou into coarse powder, put it in a closed container, and ferment the inoculated strains at 60°C for 48 hours. The strains are the composition of yeast, actinomycetes, Bacillus subtilis, and Bacillus. Use sterile distilled water to wash 3 times, filter the water and carry out sterilization treatment, add 9 times of 95% ethanol, microwave heating and reflux extraction twice, each extraction 15min, and control the reflux heating by controlling the amount of microwave radiation The temperature is 95°C, after the extraction is completed, filter and combine the extracts;

[0037] S2: Use activated carbon to decolorize the extract in step S1, then use acetone to degrease, place it in a rotary evaporator at 65°C, depressurize it to 0.02MPa, and concentrate it until the content of papaya saponin is 15%; pour it into the concentrated solution Add 85% ethanol s...

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Abstract

The invention discloses a method for extracting and purifying polyphyllin I from rhizoma paridis. The method comprises the following steps: crushing the rhizoma paridis into coarse powder and puttingthe coarse powder into a sealed container; inoculating a strain and fermenting; draining water and carrying out sterilization treatment; adding 7 to 9 times of 80 percent to 95 percent ethanol; carrying out microwave heating and reflowing extraction for 2 times; filtering and combining extracting solutions; after de-coloring and de-greasing, and decompressing and concentrating the extracting solution, continuously centrifuging and precipitating for 2 to 3 times; combing supernatant; enabling the supernatant to pass through a macroporous adsorption resin column; eluting with a 45 percent to 55percent ethanol solution and a 65 percent to 80 percent ethanol solution in sequence; collecting a 65 percent to 80 percent ethanol eluting solution and concentrating; carrying out reversed phase liquid chromatographic separation on a concentrated solution to obtain ethanol and extract; crystallizing and drying the extract to prepare the polyphyllin I. The method disclosed by the invention has relatively high extraction rate and the purity of the extracted polyphyllin I is high; the method meets the requirements for large-scale production.

Description

【Technical field】 [0001] The invention belongs to the technical field of extracting active ingredients of medicinal plants, and in particular relates to a method for extracting and purifying saponin I from the plant. 【Background technique】 [0002] Chonglou is a plant of the Liliaceae family, also known as Aescin, Jinxian Chonglou, Dengtaiqi, Iron Lampstand, Daxiu, Caoheche, Baiheche, Zhihuatou, Conchqi, Screwqi, a perennial herb, about 50 in height cm. Rhizome brown, transverse and thick, up to 3 cm thick, rough surface with nodes, fibrous roots grow on nodes. Chonglou is bitter in taste and slightly cold in nature; it is slightly poisonous and returns to the liver meridian. There are unique active ingredients in Chong Building, which have high medicinal value. There are relatively large amounts of steroidal saponins in Chonglou, and the aglycones are mainly diosgenin and pinanogenin. They have the characteristics of unique ingredients and strong pharmacological activity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 马云川
Owner 广西产学研信息科技有限公司