Glioma diagnostic and prognostic markers circ16:85633914|85634132 and its application
A technology for glioma and prognosis, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems that the survival rate of glioma patients has not been significantly improved, and the survival rate is not optimistic. Achieve the effect of convenient and accurate diagnosis and prognosis, and improve the effect of clinical treatment
Active Publication Date: 2020-06-30
XIANGYA HOSPITAL CENT SOUTH UNIV
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Problems solved by technology
At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic
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[0015] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.
[0016] 1. Research object
[0017] The tumor tissues of 30 glioma patients and 15 normal brain tissues were provided by Hunan Cancer Hospital.
[0018] 1. Extraction of RNA from Glioma / Normal Tissue
[0019] Take 0.1g of the above-mentioned tumors and normal brain tissues, wash them with appropriate amount of normal saline, put them into a mortar (the mortar should be wrapped with tinfoil in advance and baked in an oven at 180°C for 6-8 hours), add an appropriate amount of liquid nitrogen to Grind the tissue to powder, add 0.6ml Trizol (MRC Company) and continue grinding for several minutes, add 0.4ml Trizol again, transfer the mixture in the mortar to a 1.5ml enzyme-free Tube tube, and lyse on ice for 15 minutes. After the lysis, transfer to a 1.5ml enzyme-free tube, centrifuge at 12000rpm at 4°C for 10min, and transfer the supernatant to a n...
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Abstract
The invention discloses a glioma diagnosis and prognosis marker circ16:85633914|85634132 and application. A reagent for detecting annular RNAcirc16:85633914|85634132 sourced from brain tissues is usedfor preparing a diagnosis and prognosis preparation of glioma patients. The research shows that the expression of the RNA circ16:85633914|85634132 in the brain tissues of the glioma patient is apparently reduced compared with the normal brain tissues, and the ROC curve analysis shows that the RNA circ16:85633914|85634132 has relatively high diagnosis performance for the glioma. Meanwhile, the patient with relatively high expression of the circ16:85633914|85634132 has higher post-operative survival rate. The diagnosis analysis and prognosis determination can be made for the glioma high risk group by detecting the annular RNA circ16:85633914|85634132 in the glioma tissues of the glioma patient.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker used for glioma diagnosis and prognosis analysis, and a reagent for detecting the marker used to prepare glioma diagnosis and prognosis preparations, as well as a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical...
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IPC IPC(8): C12Q1/6886C12N15/113
Inventor 冯紫阳武明花李沛瑶刘强陈琼余志斌张严
Owner XIANGYA HOSPITAL CENT SOUTH UNIV