Nanostructured bactericidal composite drug, and preparation method thereof

A nanostructure and drug technology, applied in pharmaceutical formulations, antibacterial drugs, preparations for in vivo tests, etc., can solve the problems of inability to achieve location tracking and precise administration of bactericidal drugs, and achieve the effect of synergistic effect of bactericidal properties

Active Publication Date: 2018-01-26
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a nanostructured bactericidal composite drug and i

Method used

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  • Nanostructured bactericidal composite drug, and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0040] (1) Add silver nitrate solution (5mL, 20mM) and glutathione (5mL, 50mM) into 6mLs water, stir well to form GSH-Ag + complex, then add 3mL sodium borohydride aqueous solution, let it stand for 18 hours to obtain sub-nanometer silver clusters, and finally stir the silver clusters (0.5mL, 100μg / mL) with daptomycin for 8 hours, take them out and spin at 11000 rpm After 20 minutes of centrifugation for 20 minutes, the subnano-silver cluster A of surface-grafted drug molecules was obtained;

[0041] (2) Thoroughly stir chloroauric acid (5mL, 0.5mM) and CTAB (5mL, 0.2M) until evenly mixed, then add sodium borohydride (0.6mL, 0.01mM) to obtain gold nuclei, then take 1mL of the above solution and add it to CTAB-stabilized gold nanorods B were obtained by standing in 500 mL growth solution containing chloroauric acid (250 mL, 1 mM) and CTAB (9 g) for 12 h;

[0042] (3) Add the above-synthesized silver cluster A (0.5mL, 100μg / mL) and gold nanorod B (0.5mL, 1mg / mL) into 5mL of wat...

Embodiment 2

[0045] (1) Add silver acetate solution (6mL, 20mM) and glutathione (5mL, 50mM) into 6mLs water, stir well to form GSH-Ag + Complex, then add 3mL sodium borohydride aqueous solution, let it stand for 18 hours to obtain sub-nanometer silver clusters, and finally stir the silver clusters (0.6mL, 100μg / mL) with oxacillin for 8 hours, take them out and turn them at 11000 rpm After centrifugation for 20 minutes, the subnano-silver cluster A with drug molecules grafted on the surface is obtained;

[0046] (2) Thoroughly stir chloroauric acid (5mL, 0.5mM) and PSS (5mL, 0.2M) until evenly mixed, then add sodium borohydride (0.6mL, 0.01mM) to obtain gold nuclei, then take 1mL of the above solution and add it to PSS-stabilized gold nanorods B were obtained by standing in 500 mL growth solution containing chloroauric acid (250 mL, 1 mM) and PSS (18 g) for 12 h;

[0047] (3) Add the above-synthesized silver cluster A (0.6mL, 100μg / mL) and gold nanorod B (0.6mL, 1mg / mL) into 5mL of water a...

Embodiment 3

[0050] (1) Add silver oxalate solution (10mL, 20mM) and glutathione (5mL, 50 mM) into 6mLs water, stir well to form GSH-Ag + Complex, then add 6mL sodium borohydride aqueous solution, let it stand for 18 hours to obtain sub-nanometer silver clusters, and finally stir the silver clusters (0.6mL, 100μg / mL) with oxacillin for 8 hours, take them out and turn them at 11000 rpm After centrifugation for 20 minutes, the subnano-silver cluster A with drug molecules grafted on the surface is obtained;

[0051] (2) Thoroughly stir gold trichloride (4mL, 0.8mM) and PEG (5mL, 0.2M) until evenly mixed, then add sodium borohydride (0.6mL, 0.01mM) to obtain gold nuclei, then take 1mL of the above solution and add Put into a 500mL growth solution containing gold trichloride (100mL, 2.5mM) and PEG (18g) and let it stand for 12h to obtain PEG-stabilized gold nanorods B;

[0052] (3) Add the above-synthesized silver cluster A (0.8mL, 50μg / mL) and gold nanorod B (0.8mL, 1mg / mL) into 5mL of water ...

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Abstract

The invention discloses a nanostructured bactericidal composite drug, and a preparation method thereof. According to the preparation method, sub-nanometer silver clusters modified via surface graftingwith bactericidal drug molecules are taken as a bactericidal component; and chemical assembly with a gold nanorod carrier is carried out so as to obtain the stable nanostructured bactericidal composite drug. In applications of the nanostructured bactericidal composite drug, drug accurate tracing and silver cluster rapid dissociation and controllable release are realized respectively because of the dark field optical imaging and light absorption heating characteristics of gold nanorod, the synergistic interaction of sub-nanometer silver clusters with unique bactericidal performance with the drug molecules is achieved fully, and guiding significance on design of high efficiency bactericidal drugs is achieved.

Description

technical field [0001] The invention relates to the field of bactericidal compound medicine, in particular to a nanostructured bactericidal compound medicine and a preparation method thereof. Background technique [0002] Among many antibacterial drugs, nano silver has been widely used in antibacterial drugs because of its broad-spectrum antibacterial properties. At present, the bactericidal mechanism of silver can be summarized as that the silver ions dissociated from nano-silver affect cell metabolism through membrane damage, DNA damage, and active oxygen generated during the dissociation process. The study found that as the size of silver particles decreases, the surface energy increases, and the ability of the surface to quickly release silver atoms and silver ions increases, which significantly enhances the sterilization efficiency of silver particles. F.Ruiz and others found that the bactericidal activity of silver nanoparticles with different sizes was significantly ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K33/38A61K45/06A61K47/02A61K47/55A61K49/00A61P31/04
Inventor 屈军乐王鑫
Owner SHENZHEN UNIV
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