Microfluidic gene detection chip

Abstract

The invention relates to a microfluidic gene detection chip, which includes a sample loading pool, a sample dividing element and a detection element. The detection element includes a detection pool loaded with a bacteria detection agent, a detection pool loaded with a rickettsia detection agent, and a virus detection agent. The detection pool, the detection pool loaded with fungal detection agent and the detection pool loaded with biotoxin detection agent. One sample loading can detect multiple types of pathogenic microorganisms at the same time. The sample processing steps are simple, the detection efficiency is high, and the sample volume is easy to control. The volume of the liquid to be tested entering each detection pool is equal, and the detection results are more accurate, which meets the requirements of port health and quarantine. Application requirements for rapid detection and screening of pathogenic microorganisms with high efficiency and large sample volume.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a microfluidic gene detection chip. Background technique [0002] Most of the highly pathogenic pathogenic microorganisms have the characteristics of strong infectivity, fast transmission, short incubation period and acute onset. threaten. At present, some highly pathogenic pathogenic microorganisms have crossed the barrier between species, and irregular outbreaks in humans have become an increasingly common phenomenon. Due to the complexity and mobility of port health and quarantine objects, and the unknown and variability of potentially highly pathogenic pathogenic microorganisms, it is very necessary to quickly detect highly pathogenic pathogenic microorganisms. [0003] Traditional detection methods mainly include direct smear microscopy, isolation and culture, etc. However, microscopic inspection methods relying on in vitro culture of pathogenic microorganism...

AI Technical Summary

Problems solved by technology

However, the problem with the traditional immunoassay technology is that it is difficult to detect the window period of pathogenic microorganism infection. Even if the infected person has been infected with the virus, due to the small number of virus copies and the low abundance of virus antibodies, the results of checking the virus antibody are often missed diagnosis

Real-time fluorescence quantitative PCR technology is currently the mainstream method for the detection of nucleic acid molecules of pathogenic microorganisms. This method has strong specificity, but only one pathogenic target can be detected in one experiment, and the detection throughput is low. It is not very fast to detect a large number of pathogenic targets at the same time cope well

These two methods often take a long time to detect a variety of pathogenic microorganisms, which also delays the best time for diagnosis and treatment

[0004] In summary, the traditional detection products are not highly automated, the detection efficiency is low, and the detection results are inaccurate

It is far from meeting the application requirements of high-efficiency port health quarantine and rapid detection and screening of pathogenic microorganisms with a large sample size

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