Chemiluminescence immunoassay disk based on centrifugal microfluidics technology and working method thereof

A technology of chemiluminescent immunity and microfluidic technology, which is applied in the field of microfluidics, can solve the problems of large amount of reagents and reduce the cost of user detection, and achieve the effect of simple structure, reduced complexity and reduced cost

Pending Publication Date: 2018-01-30
ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing tubular chemiluminescence uses a relatively large amount of reagents. For chemiluminescence with high reagent costs, reducing the amount of reagents used can significantly reduce the detection cost of users, especially for grassroots medical institutions, which need to be miniaturized and simplified. inspection equipment

Method used

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  • Chemiluminescence immunoassay disk based on centrifugal microfluidics technology and working method thereof
  • Chemiluminescence immunoassay disk based on centrifugal microfluidics technology and working method thereof
  • Chemiluminescence immunoassay disk based on centrifugal microfluidics technology and working method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Figure 1-2 A schematic diagram of the structure of the chemiluminescent immunodisc based on the centrifugal microfluidic technology in the embodiment of the present invention is given schematically, as shown in Figure 1-2 As shown, the disc includes:

[0030] At least two detection parts, the detection part includes:

[0031] Sample port 1, first reagent port 2, detection tank 10 and waste liquid tank 12, the waste liquid tank has an exhaust hole 13; the minimum radius of rotation R of the waste liquid tank 3 with the detection slot’s maximum rotation radius R 2 The relationship between is: R 3 -R 2 <0; the volume of the detection tank is 10-400 μL, and the volume of the waste liquid tank is 200-3000 μL;

[0032] Valve 11, the valve 11 controls the communication between the detection tank 10 and the waste liquid tank 12; the valve includes a tortuous channel formed in the disc, and the width of the channel is 50-1000 μm , one end of the channel communicates with...

Embodiment 2

[0049] An application example of the chemiluminescent immunodisc based on centrifugal microfluidic technology and its working method in the detection of human chorionic gonadotropin standard according to Example 1 of the present invention.

[0050] The working method includes the following steps

[0051] 1. Use a chemiluminescent immunodisc for twelve people, add 0, 5, 20, 100, 500, 2500, 5500 IU of standard substances, add magnetic bead antibody, and enzyme-labeled antibody in succession. Add magnetic bead antibody, the volume is between 1 and 100 microliters, typically 20 microliters; add enzyme-labeled antibody, the volume is between 1 and 100 microliters, typically 20 microliters; start the motor, and the disk rotates With a typical speed of 500 rpm, the reagents are centrifuged into an incubation tank for incubation.

[0052] 2. After incubation, continue to centrifuge. The typical speed of the disc rotation is 1500 rpm. Centrifuge the mixed solution into the mixing tank...

Embodiment 3

[0061] An application example of the chemiluminescent immunodisc based on centrifugal microfluidic technology and its working method in PCT detection of procalcitonin according to Example 1 of the present invention.

[0062] In this application example, the difference from the above-mentioned embodiment 2 is that in the cleaning process, the number of times of cleaning should be increased to achieve the cleaning effect of the PCT item.

[0063] In this application example, the selected PCT standard is also selected, and the concentrations of the selected PCT standard are 0, 0.05, 0.2, 1, 5, 25, and 100ng / ml respectively. The measured results of the PCT standard are as follows:

[0064]

[0065] Such as Figure 4 As shown, according to the test results of the chemiluminescent immunodisc of the present invention on the PCT standard, each concentration can be opened in a gradient, and the efficiency is high and the repeatability is good.

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Abstract

The invention provides a chemiluminescence immunoassay disk based on a centrifugal microfluidics technology and a working method thereof. The chemiluminescence immunoassay disk based on the centrifugal microfluidics technology comprises a sample loading port, a first reagent port, a detecting groove and a waste liquid groove, and a valve, the valve controls communication between the detecting groove and the waste liquid groove, and comprises a meandering passage formed in the disk, a relationship between a minimum rotation radius R1 of the passage and a maximum rotation radius R2 of the detecting groove is that R1-R2 is less than 0. The chemiluminescence immunoassay disk based on the centrifugal microfluidics technology has the advantages of stability, reliability, efficiency, quickness and the like.

Description

technical field [0001] The invention relates to microfluidics, in particular to a chemiluminescent immune disc based on centrifugal microfluidics technology and a working method thereof. Background technique [0002] Chemiluminescent immunoassay is a type of immunoassay method in which chemiluminescent reagents directly label antibodies or antigens. It has the advantages of high sensitivity, strong specificity, no radiation, long-term effective markers, and full automation. Chemiluminescent immunoassay methods are diverse and applicable to a wide range of applications. They have been applied to the immunoassay of antigens, haptens, and antibodies. They are fast and effective, and their linear range is relatively wide, which meets the needs of clinical testing. [0003] At present, the popular automatic tube-type luminescent detection process is as follows: 1. Complete the mixed incubation of the sample, magnetic bead antibody, and enzyme-labeled antibody in the detection tub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/532
Inventor 程林金烨琦章诗校余波
Owner ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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