Method for transmission electron microscope observation of phytoseiulus persimilis reproductive system

Abstract

The invention relates to a method for transmission electron microscope observation of a phytoseiulus persimilis reproductive system. The method is characterized in that the phytoseiulus persimilis reproductive system is obtained prior to preparation of a transmission sample. The method specifically includes steps: acquiring male and female phytoseiulus persimilis in mating, and performing lactic acid soaking, dehydrating and drying treatment to finally obtain the phytoseiulus persimilis reproductive system; preparing the transmission sample of the reproductive system by seven steps including fixing, dehydrating, embedding, curing, slicing, dyeing and observing in sequence. By adoption of the simple and ingenious method, the phytoseiulus persimilis reproductive system is obtained for the first time, transmission electron microscope observation of an ultrastructure of the reproductive system is realized for the first time, and acquisition of the reproductive system provides raw materialsfor transmission electron microscope observation of the structure of the reproductive system, and transmission electron microscope observation results are beneficial to researching of reproductive mechanisms.

Description

technical field [0001] The invention relates to a transmission electron microscope observation method for the reproductive system of the phytoseiid mite, and belongs to the field of basic biological research of the phytoseiid mite. Background technique [0002] The reproduction of Phytoseius militaris is special. If the female mite does not mate, it will not lay eggs, and it is believed that all offspring develop from fertilized eggs; while the female mite is diploid, and the male mite is haploid. This result is considered to be determined by the special reproductive mode of Phytoseius militaris, that is, pseudo-androgenetic reproduction. However, this conclusion is self-contradictory when it is demonstrated. In addition, whether it is a newly laid egg or a laparotomy egg, the chromosomes are all haploid. Obviously, if the chromosome is lost or heterogeneous, it must also occur before the egg is laid, or even before or after the egg cell is just developed. Therefore, the r...

AI Technical Summary

Problems solved by technology

[0004] However, due to the small size and fragility of Phytoseius militaris, the body is filled with opaque body fluids, it is impossible to accurately locate its reproductive system, and a little carelessness will lead to fragmentation of the worm body. There is no relevant information on how to dissect and how to Observation of its reproductive system has been reported, so how to obtain and conduct transmission electron microscopy observation of the reproductive system of Phytoseiillus chileensis remains to be resolved