Method for transmission electron microscope observation of phytoseiulus persimilis reproductive system
A technology of Phytoseiid mite and reproductive system in Chile, which is applied in material analysis using radiation, material analysis using wave/particle radiation, sampling, etc. It can solve the problems of broken body, inability to accurately locate the reproductive system, full of body fluids, etc.
Inactive Publication Date: 2018-02-02
SAFE BEST & NECESSARY BEIJING BIOTECH CO LTD
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The invention relates to a method for transmission electron microscope observation of a phytoseiulus persimilis reproductive system. The method is characterized in that the phytoseiulus persimilis reproductive system is obtained prior to preparation of a transmission sample. The method specifically includes steps: acquiring male and female phytoseiulus persimilis in mating, and performing lactic acid soaking, dehydrating and drying treatment to finally obtain the phytoseiulus persimilis reproductive system; preparing the transmission sample of the reproductive system by seven steps including fixing, dehydrating, embedding, curing, slicing, dyeing and observing in sequence. By adoption of the simple and ingenious method, the phytoseiulus persimilis reproductive system is obtained for the first time, transmission electron microscope observation of an ultrastructure of the reproductive system is realized for the first time, and acquisition of the reproductive system provides raw materialsfor transmission electron microscope observation of the structure of the reproductive system, and transmission electron microscope observation results are beneficial to researching of reproductive mechanisms.
Preparing sample for investigationMaterial analysis by transmitting radiation
UltrastructureObservation method +6
- Experimental program(1)
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