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Biological prevention and control method of longhorn beetles

A biological control and beetle technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems such as no reports of Beauveria bassiana and sodium chloride insecticides, and achieve prevention and control Significant effect, good control effect, environment-friendly effect

Active Publication Date: 2018-02-09
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the application of insecticides combining Beauveria bassiana and sodium chloride in the control of beetles

Method used

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  • Biological prevention and control method of longhorn beetles
  • Biological prevention and control method of longhorn beetles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Indoor virulence assay experiment of Beauveria bassiana and sodium chloride compounded longhorn beetle

[0018] During indoor toxicity determination, 10 g of Beauveria bassiana powder was weighed and dissolved in 2L of water, and mixed evenly to obtain a Beauveria bassiana solution. The used Beauveria bassiana powder contains 20 billion spores of Beauveria bassiana / g. Measure 11 parts of Beauveria bassiana solutions of 100ml each from 2L of Beauveria bassiana solution, and then add NaCl to them respectively, so that the concentrations of NaCl in 9 parts of Beauveria bassiana solutions are: 0mmol / L, 25mmol / L, 50mmol / L, 75mmol / L, 100mmol / L, 125mmol / L, 150mmol / L, 200mmol / L, 250mmol / L, 300mmol / L, 400mmol / L, sequentially obtained Beauveria bassiana · sodium chloride aqueous dispersion solution, wherein Zombies have a spore concentration of 1×10 8 pcs / mL -1 .

[0019] The toxicity of Beauveria bassiana solution, Beauveria bassiana·NaCl aqueous dispersion so...

Embodiment 2

[0022] Embodiment 2: Beauveria bassiana·sodium chloride water dispersant is to the indoor toxicity determination experiment of longicorn beetle

[0023]Take by weighing Beauveria bassiana powder and sodium chloride total 30g (wherein Beauveria bassiana powder and sodium chloride mass ratio are respectively: 5:1.46; 5:2.92; 5:4.38; 5:5.84; 5:7.30; 5:8.77 ; 5:11.69; 5:14.61g; 5:17.53; 5:23.38, the Beauveria bassiana powder used contains 20 billion spores of Beauveria bassiana / g.), wetting agent pulls open powder 8g, dispersant sodium lignosulfonate 18g, disintegrant calcium chloride 5g, stabilizer disodium hydrogen phosphate 2g, binder sodium polyacrylate 5g, carrier diatomaceous earth 32g, the above raw materials are put into mixing by the preparation method of water-dispersible granules (1, according to formula The raw materials are mixed evenly; 2. After being mixed evenly, they are sanded by a sand mill and tested to be qualified before spraying granulation; 3. After sprayin...

Embodiment 3

[0032] During the indoor toxicity test, weigh 1 g of Beauveria bassiana powder and 7.5 g of sodium chloride, dissolve them in 1 L of water, and mix them uniformly to obtain a Beauveria bassiana solution. The used Beauveria bassiana powder contains 20 billion spores of Beauveria bassiana / g.

[0033] The indoor toxicity test was carried out according to the method of Example 1, and the results showed that the Beauveria bassiana solution of the present example had a better killing effect on the beetle beetle.

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Abstract

The invention discloses a biological prevention and control method of longhorn beetles. The method comprises the following steps: dissolving a medicament containing pathogenic microorganisms for preventing and controlling longhorn beetles into water to prepare a water dispersing agent, and then contaminating the longhorn beetles with the water dispersing agent, wherein the medicament containing the pathogenic microorganisms for preventing and controlling the longhorn beetles comprises beauveria bassiana and sodium chloride, and the ratio of the number of the beauveria bassiana spores to the grams of the sodium chloride is: (1-8)*200 billion:(2.9-7.5 g). According to a test provided by the invention, a remarkable synergistic effect is obtained by compounding the beauveria bassiana and the sodium chloride, the prevention and control effect is very good, and the prevention and control effect of the serious Dancong tea longhorn beetles at present is obvious. In addition, the compounded medicament has the characteristics of friendliness to environment, and no pollution to soil or underground water resources, has a simple using method, and is easy to promote and apply, so that the insecticidal effect of the pathogenic microorganisms is improved, and the tea leaves are free of pollution.

Description

Technical field: [0001] The invention belongs to the field of prevention and control of longicorn beetles, and in particular relates to a biological prevention and control method of longicorn beetles. Background technique: [0002] Beauveria bassiana: Beauveria bassiana is an ascomycete entomogenic fungus. The high sporopollen of Beauveria bassiana is non-toxic and tasteless, and has no environmental pollution. It has persistent infection power to pests, and once the pests are infected, they can spread continuously. No pesticide residue, even if the product is harvested immediately after the application of Beauveria bassiana, it will not cause any pesticide residues; no resistance, Beauveria bassiana is infected and killed by contact with the body wall of the pest under natural conditions, and the pest will not develop any resistance to it Re-growth, Beauveria bassiana new biopesticide contains living fungi and spores, after being applied to the field, with the help of appro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N59/08A01P7/04
CPCA01N59/08A01N63/30Y02A50/30
Inventor 李军韩诗畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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