A method for detecting alpha-fetoprotein based on graphene, thionine and nucleic acid aptamer
A technology for alpha-fetoprotein and graphene, applied in the field of detecting alpha-fetoprotein, can solve the problems of complicated operation, high cost and high technical requirements, and achieve the effects of simple synthesis, low cost and stable performance
Active Publication Date: 2019-10-18
GUILIN UNIV OF ELECTRONIC TECH
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[0003] The technical problem to be solved by the present invention is to provide a method for detecting AFP based on graphene, thionine and nucleic acid aptamer, which solves the problems of high cost, complicated operation and high technical requirements of traditional AFP detection methods
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[0040] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0041] A method for detecting AFP based on graphene, thionine and nucleic acid aptamer, the detection principle see figure 1 ,
[0042] The implementation steps are as follows:
[0043] 1. Electrode pretreatment: Before using the screen-printed electrode, first place it in 0.5 mol / L H 2 SO 4 In the solution, use the cyclic voltammetry scanning method to scan, the scanning voltage is -0.2 V ~ 1.0 V, and scan 10 times within this range; after the scanning is completed, the electrode is washed with distilled water and dried for later use;
[0044]2. Electrode modification and aptamer immobilization: Put the activated screen-printed electrode into 5 mL 0.01% HAuCl 4 In the solution, the electrodeposition was carried out while stirring with a magnetic stirrer, the deposition time was 120 s, and the deposition potential was -0.5 V. After the de...
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The invention discloses a method used for detecting alpha fetoprotein based on graphene, thionine, and a nucleic acid aptamer. According to the method, electrodeposition technology is adopted for deposition of nano-gold on the surface of a screen-printed electrode, electrostatic adsorption is adopted for absorption of graphene and thionine which drop on an electrode surface onto the surface of thescreen-printed electrode, thionine is taken as a bridge molecule for capturing of the nucleic acid aptamer onto the surface of a modified electrode. A nanometer aptamer biosensor can be constructed based on the extremely large specific surface area and signal amplification effect of graphene, the high loading capacity of thionine on the aptamer, and the specific recognition effect of the aptameron alpha fetoprotein. Peak value current is measured through differential pulse voltammetry, and the relation curve of the current to the alpha fetoprotein concentration is drawn, so that detection onalpha fetoprotein is short in time, low in cost, and high in the specificity.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting alpha-fetoprotein. Background technique [0002] Alpha-fetoprotein (Alpha-fetoprotein, AFP) is a glycoprotein of the albumin family, which is a single polypeptide chain formed by more than 590 amino acid residues. The content of alpha-fetoprotein in normal adults is very small, but in liver cancer The content of alpha-fetoprotein in the patient's body is related to the degree of differentiation of hepatocellular carcinoma. AFP detection methods mainly include enzyme-linked immunosorbent assay, fluorescent immunoassay, electrochemiluminescence and electrochemical immunosensor. However, conventional immunoassays have their inherent disadvantages, such as radioactive contamination in enzyme-linked immunoassays, the activities of enzymes and antibodies used in immunoassays are easily affected by external temperature and pH, unstable on...
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IPC IPC(8): G01N27/48
Inventor 李桂银李珊珊薛叶薇黄勇梁晋涛王志宏董辰杨周治德
Owner GUILIN UNIV OF ELECTRONIC TECH