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Method for carrying out separation and purification on algae species of euglenophyta by adopting flat-panel solid culture medium

A solid medium, separation and purification technology, applied in the field of purification of microalgae species, can solve the problems of induced mutation, difficult survival, complicated operation, etc., and achieve the effects of improving the probability of success, simple separation and purification, and shortening the growth cycle

Inactive Publication Date: 2018-02-13
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported algae sterilization methods mainly include microscope picking algae cells and adding antibiotics to algae liquid to eliminate bacteria. It is difficult to cultivate and difficult to survive
The latter directly adds antibiotics to the algae liquid. Although it can effectively inhibit the bacteria in the algae liquid, it will affect the growth of algae and even induce mutations if it cannot be completely eliminated.

Method used

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  • Method for carrying out separation and purification on algae species of euglenophyta by adopting flat-panel solid culture medium
  • Method for carrying out separation and purification on algae species of euglenophyta by adopting flat-panel solid culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for separating and purifying euglena species from a plate solid medium specifically comprises the following steps:

[0037] (1) Take 1.0 L of unseparated and purified euglena liquid with bacterial contamination (it was found to grow poorly during the cultivation process, and flocculation and precipitation occurred in the algae culture liquid);

[0038] (2) centrifuging the euglena liquid, the centrifuging steps are:

[0039] The first step is to divide 1.0L of algae liquid into several 50ml centrifuge bottles;

[0040] The second step is to perform centrifugation, centrifugation conditions: 3000rpm, 2.0min;

[0041] The third step is to pour off the upper liquid, add 20.0mL sterilized physiological saline to the centrifuge bottle with precipitated algae mud, and vortex to mix;

[0042] The fourth step, continue the centrifugation operation, the centrifugation conditions are the same as above;

[0043] Step 5: Repeat steps 3 to 4 twice;

[0044] The sixth ...

Embodiment 2

[0059] The method for separating and purifying euglena species from a plate solid medium specifically comprises the following steps:

[0060] (1) Take 1.0 L of unseparated and purified euglena liquid with bacterial contamination (it was found to grow poorly during the cultivation process, and flocculation and precipitation occurred in the algae culture liquid);

[0061] (2) centrifuging the euglena liquid, the centrifuging steps are:

[0062] The first step is to divide 1.0L of algae liquid into several 50ml centrifuge bottles;

[0063] The second step is to carry out centrifugation operation, centrifugation conditions: 5 000rpm, 3.0min;

[0064] The third step is to pour off the upper liquid, add 20.0mL sterilized physiological saline to the centrifuge bottle with precipitated algae mud, and vortex to mix;

[0065] The fourth step, continue the centrifugation operation, the centrifugation conditions are the same as above;

[0066] Step 5: Repeat steps 3 to 4 twice;

[006...

Embodiment 3

[0082] The difference from Example 1 is that the antibiotic in step (5) is oxytetracycline with a concentration of 70ppm.

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Abstract

The invention discloses a method for carrying out separation and purification on algae species of euglenophyta by adopting a flat-panel solid culture medium, and relates to the technical field of purification of algae species of microalgae. The method comprises the following steps: firstly, centrifuging algae solutions of euglenophyta which is polluted by bacteria and is not subjected to separation and purification, cleaning euglenophyta slurry and removing the culture medium and physiological metabolism substances; then, coating a resistant flat panel added with composite antibiotics with thediluted algae solutions; then further drawing a line and carrying out separation and purification by adopting the resistant flat panel, then performing batch micro-culture by adopting an aseptic 96-hole cell culture plate, transferring into a triangle bottle and carrying out subsequent enlarged culture. By utilizing the method disclosed by the invention, the algae species of euglenophyta are separated, and the method has the advantages of easiness for operation, high efficiency, low cost and the like; the algae species with higher purity can be obtained in a short time, growth periods are shortened, and the probability of success of large-scale growth is improved, so that the aims of increasing the yield and reducing the cost are fulfilled.

Description

technical field [0001] The invention relates to the technical field of purification of microalgae species, in particular to a method for separating and purifying euglena species on a flat solid medium. Background technique [0002] Euglena is an intermediate organism between animals and plants. It has the protein composition of animals and plants and is a good bait organism. In 2013, my country approved Euglena as a new food raw material, which makes Euglena Has a very good market prospect. In recent years, Japan and other countries have successively developed patented products of euglena as fish bait. my country started relatively late in this field, and the relevant technologies are still immature, resulting in a relatively low level of industrialization development. Among them, euglena is very easy to be infected with bacteria in the process of subculture and large-scale cultivation of algae, resulting in insufficient continuity of production, unstable product quality, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李悦明周文礼许丽娜张文慧夏修峦徐建春范栋
Owner QINGDAO KEHAI BIOLOGICAL
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