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Tylophora ovata rooting culture medium and preparing method thereof

A rooting medium and baby vine technology, applied in the direction of medium, botany equipment and methods, planting substrate, etc., can solve the problems of low natural pollination rate of wild resources, destruction of growth environment, high price and other problems

Inactive Publication Date: 2018-02-16
融安县辉煌农产品种养专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Waerteng is used for cancer pain relief among the people in Guangxi, and it has good curative effect. Due to years of excavation, the natural pollination rate of wild resources is low, and the traditional growth environment has been destroyed.
The use of tissue culture technology to serially cultivate sages has become one of the important breeding methods for improved varieties. At present, there are few researches on the tissue culture of scorpions, and there is a lack of a medium that can effectively promote the rooting of tissue cultured plantlets of S. spp.

Method used

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  • Tylophora ovata rooting culture medium and preparing method thereof
  • Tylophora ovata rooting culture medium and preparing method thereof

Examples

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Embodiment 1

[0020] The preparation method of the copper money grass liquid is as follows: take the copper money grass, wash the root with water, cut the root, soak it with 85% alcohol for 10-15 minutes, take it out, add the same weight of sterile water, break it up and stir, and filter it through filter paper. have to.

[0021] It can be understood that the soaking time in 85% alcohol in this embodiment can also be any value in 10min, 15min or 10-15min, which can achieve the purpose of the present invention.

Embodiment 2

[0023] The preparation method of the pitaya juice is as follows: take the pitaya pulp and seeds, put them into a high-speed blender and stir, take them out and filter them with a 400-mesh filter cloth, centrifuge for 5-10 minutes in a centrifuge, and take the upper layer to obtain the pitaya juice.

[0024] It can be understood that, in this embodiment, the centrifuge centrifuges for 5 minutes, 10 minutes or any value of 5-10 minutes, which can achieve the purpose of the present invention.

Embodiment 3

[0026] A method for the rooting culture medium of vines, comprising the following steps: according to the weight ratio, take the sweet potato puree and dissolve it with 1 / 3 of the total water and stir it, and then add jasmonic acid, 6-benzylaminopurine, and indole while stirring. Indole butyric acid, ethephon, brassinosterol, hydrolyzed milk protein, copper money grass liquid, pitaya juice, vitamin B6, and continue to stir for 20 minutes to obtain solution I; then take the modified White medium formula and dissolve it with 1 / 3 of the total water volume , and then mix with solution I evenly, add agar, adjust the volume to the required scale with water, adjust the pH value between 5.7, continue to stir for 15 minutes, fill, and then put the medium into sterilized under the conditions of 120°C and 0.22MPa After 25 minutes, take it out and let it cool down to obtain the rooting medium of the vine.

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Abstract

The invention relates to the technical field of seed treatment, in particular to a tylophora ovata rooting culture medium and a preparing method thereof. The tylophora ovata rooting culture medium isprepared in the mode that agal-agal with the density of 3-7 g / L, sweet potato puree with the density of 20-30 mg / L, jasmonic acid with the density of 10-18 mg / L, 6-benzylaminopurine with the density of 1-4 mg / L, indolebutyric acid with the density of 2-6 mg / L, ethephon with the density of 1-4 mg / L, brassinosteroid with the density of 4-9 mg / L, lactoalbumin hydrolysate with the density of 4-12 mg / L, hydrocotyle verticillata solution with the density of 8-16 mg / L, dragon fruit juice with the density of 6-14 mg / Land vitamin B6 with the density of 0.4-1.2 mg / L are added to a White improved culturemedium. The tylophora ovata rooting culture medium can promote the rooting speed and number of tissue culture seedlings of tylophora ovata, improve the adaptive capacity of the tissue culture seedlings, and improve the survival rate of the tissue culture seedlings after transplantation; the invention further provides a preparing method of the tylophora ovata rooting culture medium.

Description

【Technical field】 [0001] The invention relates to the technical field of medium for growing seedlings of plants, in particular to a medium for rooting vines and a preparation method thereof. 【Background technique】 [0002] Tylophora is a perennial grapelike vine-like herb of the genus Tylophora in the family Asclepiadaceac. It is mainly used as medicine with roots and whole plants. It is mainly grown in Yunnan, Guangxi, Guangdong, Hunan, Jiangxi, Zhejiang, Taiwan, Vietnam, Laos, Myanmar, India and other places like warmth, can tolerate shade, and are relatively cold-tolerant. It often grows in mountainous bushes below 900 altitudes, or in valleys and sunny dense forests. The baby's vines are winding, suitable for fences, railings, door frames, and flower galleries, and can also be dotted in the gaps in rockery and rock slopes. Climbing around them, the vines are drooping, which is quite unique. [0003] Waerteng has a long history of being used as medicine in our country. "...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01G24/22A01G24/30
Inventor 杜乐贵
Owner 融安县辉煌农产品种养专业合作社