Ultrahigh-sensitivity up-conversion nano temperature probe and preparation method thereof

Abstract

The invention provides a novel ultrahigh-sensitivity nano fluorescent temperature probe material KMn<0.8>Yb<0.19>Er<0.01>F<3>. Because the electron-state wave function of Mn<2+> ions and Er<3+> ions of a matrix are overlapped, through an exchange effect, the <4>T<1> energy level of the Mn<2+> ions builds an electron coupling channel for the <2>H<11 / 2> and <4>S<3 / 2> energy levels of the Er<3+> ionswith the <4>F<9 / 2> energy level of the Er<3+> ions. The exchange effect distance is regulated and controlled depending on the temperature, the intensity ratios of green light emitting bands at the positions of 522 nm and 542 nm to the red light emitting bands at a position of 654 nm of the Er<3+> ions are remarkably changed, so that temperature detection suitable for a temperature range in livingbodies is achieved. The monitoring emission peak distance of the up-conversion fluorescent nano temperature probe material is large and reaches 112 nm, the signal discrimination degree is high, the absolute temperature sensitivity is 0.0113K<-1>, the highest relative temperature sensitivity is 5.7%K<-1>, the temperature sensitivity of the up-conversion fluorescent nano temperature probe materialexceeds the temperature sensitivity of other up-conversion fluorescent nano temperature probe materials which are currently studied by far. Therefore, after surface functionalization, the up-conversion fluorescent nano material can be used as a fluorescent nano temperature probe suitable for temperature detection in living bodies.

Description

technical field [0001] The invention relates to the fields of rare earth-doped up-conversion luminescent nanomaterials, temperature detection technology and the like. Background technique [0002] Cellular activities in organisms, such as cell division, gene expression, and metabolism, are actually achieved through endothermic or exothermic chemical reactions, usually accompanied by temperature changes. In this regard, the realization of intracellular temperature detection is of great significance for revealing many cellular activities, promoting the development of cell biology, and realizing the diagnosis and treatment of diseases at the cellular level. The unique environment inside the cell makes the traditional contact temperature measurement method unable to meet the temperature measurement requirements. Thanks to the great achievements of inorganic nanoscience and technology, the nanofluorometer thermology developed in recent years has the advantages of remote observat...

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Problems solved by technology

On the other hand, by S r =ΔE / kT 2 It can be seen that a smaller ΔE value will lead to lower relative temperature sensitivity, and at the same time make the partial overlap of the monitoring emission peaks, resulting in poor signal discrimination

Therefore, within the temperature detection range of biological applications, it is difficult for this temperature measurement technology to obtain high absolute temperature sensitivity and high relative temperature sensitivity at the same time, which has become an inevitable inherent defect of this temperature measurement technology.

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