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Double-excitation fluorescence probe preparation and application in detection of hydrazine in water

A fluorescent probe, fluorescein technology, applied in the field of analytical chemistry, can solve problems such as liver central nervous system damage, and achieve the effect of low cost

Active Publication Date: 2018-02-16
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, if a person is exposed to hydrazine for a long time, it can cause fatal damage to the liver, kidneys and central nervous system

Method used

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  • Double-excitation fluorescence probe preparation and application in detection of hydrazine in water
  • Double-excitation fluorescence probe preparation and application in detection of hydrazine in water
  • Double-excitation fluorescence probe preparation and application in detection of hydrazine in water

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 Novel dual excitation fluorescent probe Tb III -dtpa-bis(fluorescein)

[0035] (1) Preparation method

[0036] 1, the preparation of diethylenetriaminepentaacetic dianhydride (dtpaa)

[0037] Weigh 7.8100g (0.02mmol) of dtpa, 16.0mL (0.08mmol) of acetic anhydride, and 10.0mL (0.12mmol) of pyridine into a three-necked round bottom flask, mix well, and heat with stirring at 65°C for 24h. Cool to room temperature, filter the reaction mixture, wash twice with a small amount of acetic anhydride and anhydrous ether, filter with a vacuum pump, and dry the resultant in a vacuum oven at 80°C to obtain dtpaa.

[0038] 2. Preparation of diethylenetriaminepentaacetic acid difluorescein (dtpa-bis (fluorescein))

[0039]Take 1.9610g of dtpaa (5.5mmol), 8.0mL (16.5mmol) of triethylamine, anhydrous DMF (50mL), and 1.6515g (11mmol) of fluorescein in a three-neck round bottom flask, and mix well. Heating at a constant temperature of 100°C and stirring rapidly for 24 hour...

Embodiment 2

[0047] Embodiment 2 Double excitation fluorescent probe Tb 3+ -Application of dtpa-bis(fluorescein) in detection of hydrazine

[0048] 1. Fluorescence spectra of dual-excitation fluorescent probe hydrazine detection

[0049] Experimental conditions: Take a certain amount of hydrazine (N 2 h 4 ) was prepared with distilled water to a concentration of 1.0×10 -3 mol / L solution as a hydrazine stock solution.

[0050] Take 5.0 mL of the hydrazine stock solution in a 50 mL volumetric flask, and then add 5.0 mL of the dual-excitation fluorescent probe stock solution prepared in Example 1, and use buffer to make up the volume. At this time, the probe concentration was 5.0×10 -5 mol / L, the concentration of each detected substance is 1.0×10 -4 mol / L. Changes in fluorescence spectra were observed at excitation wavelengths of 247 nm and 500 nm.

[0051] The result is as Figure 5a , Figure 5b shown. Such as Figure 5a As shown, under the excitation of 247nm, the fluorescent p...

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PUM

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Abstract

The invention discloses a double-excitation fluorescence probe and a preparation method and application thereof in detection of hydrazine in water. The preparation method includes: taking diethylenetriamine pentaacetic acid, acetic anhydride and pyridine, stirring and heating for 24h at 65 DEG C, cooling, filtering, washing and drying; well mixing obtained diethylenetriamine pentaacetic acid dianhydride with triethylamine, DMF (dimethyl formamide) and fluorescein, heating and stirring for 24h at a constant temperature of 100 DEG C, cooling, filtering, and drying; dissolving obtained diethylenetriamine pentaacetic acid-bis(fluorescein) into Tris-HCl / DMSO (10mmol.L<-1>, v / v=9:1) buffer solution with pH equal to 7.5, mixing with Tb(NO3)3.6H2O, and heating or placing for more than two hours toobtain a target product. Uric acid detection is realized by combination of Tb<3+>-dtpa-bis(fluorescein) as a double-excitation probe and a fluorescence method. The method is simple, novel, high in efficiency and low in cost, and applicability to practical water samples is realized.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to the synthesis of novel fluorescent probes and the detection of hydrazine. Background technique [0002] Hydrazine is an important industrial raw material and should be widely used in the fields of agriculture, medicine and chemistry. Because of its flammable and explosive properties, hydrazine is also a familiar high-energy fuel used in rocket propellants. From the chemical structure point of view, hydrazine has two functional groups, and both can show nucleophilic properties, which makes hydrazine can synthesize many derivative products, such as antidiabetic drugs, anti-tuberculosis drugs, plant growth regulators, herbicides, slow etchants and dyes. More recently, a common hydrazine derivative has also been discovered, such as hydralazine, which is used in the treatment of heart failure and high blood pressure. Despite its wide range of applications, we should be highly aw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07F5/00G01N21/64
CPCC07F5/00C09K11/06C09K2211/182G01N21/64
Inventor 宋有涛任佩佩刘冠宏杨帆王君
Owner LIAONING UNIVERSITY
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