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PCR-SSCP primers for detecting UCP1 gene mutation, and applications of PCR-SSCP primers in yak meat quality trait identification methods

A meat quality, yak technology, applied in the field of molecular biology, can solve problems such as affecting carcass quality, and achieve the effects of sensitive response, strong specificity and simple operation

Active Publication Date: 2018-02-16
GANSU AGRI UNIV
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Problems solved by technology

Hou Guanyu et al found that calpain 3 (CAPN3) affects carcass quality and there is a significant difference (P<0.05)

Method used

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  • PCR-SSCP primers for detecting UCP1 gene mutation, and applications of PCR-SSCP primers in yak meat quality trait identification methods
  • PCR-SSCP primers for detecting UCP1 gene mutation, and applications of PCR-SSCP primers in yak meat quality trait identification methods
  • PCR-SSCP primers for detecting UCP1 gene mutation, and applications of PCR-SSCP primers in yak meat quality trait identification methods

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Source of materials:

[0036] 1.1 Test cattle:

[0037] In this experiment, three yak groups were selected as the research objects. There were 722 Gannan yaks, of which 432 Gannan yaks were tested for their carcass and meat quality traits.

[0038] All yak groups were taken blood samples, 10mL of blood was collected from the jugular vein of each cow, anticoagulated with sodium citrate-dextrose (ACD), and stored at -70°C; some yak blood samples were dropped on FTA cards (Flinders technology Associates, FTA ) (Whatman, Middlesex, UK) stored at room temperature.

[0039] 1.2 Reagents used and sources:

[0040] TaKaPa Premix Taq polymerase (Beijing Biotech Biotechnology Co., Ltd.), Goldview nucleic acid dye, Tris equilibrated phenol, chloroform, proteinase K, ddH 2 O, citric acid, sodium citrate, PBS buffer, DNA Marker, isoamyl alcohol, absolute ethanol, 75% alcohol, 10% SDS, agarose (Agrose), N,N'-methylenebisacrylamide, Disodium ethylenediamine tetraacetate (EDTA)...

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Abstract

The present invention discloses two pairs of PCR-SSCP primer pairs for detecting UCP1 gene mutation, wherein the two PCR-SSCP primer pairs respectively are P5 and P6, and the nucleotide sequences of the forward primers and the reverse primers are represented by SEQ ID NO.1-4 in the sequence table. The invention further provides applications of the two PCR-SSCP primer pairs in yak meat quality trait prediction and identification methods, an identification method, and a corresponding kit. According to the present invention, the primers P5 and P6 are designed according to the UCP1 gene sequence of common cattle, the UCP1 gene of yak is amplified, the sequence and the population genetic characteristics of the UCP1 gene detection region are analyzed, the Gannan yak meat production performance determination data is combined, the influence of the UCP1 gene mutation on the carcass and the meat quality traits is analyzed, and the important economic trait candidate gene molecule genetic study data of yak is enriched, such that the meat quality traits of yak are predicted, and the advantages of sensitive reaction, strong specificity, easy operation, high precision and rapid detection are provided.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a PCR-SSCP primer for detecting UCP1 gene mutation and its application in methods for predicting and identifying yak meat quality traits. Background technique [0002] Yak (Bos grunniens) is a unique breed of cattle on the Qinghai-Tibet Plateau and its adjacent alpine and subalpine regions. There are more than 14 million yaks in China, accounting for more than 90% of the total number of yaks in the world, mainly in the alpine pastoral areas of Tibet, Qinghai, Sichuan, Gansu, Yunnan and Xinjiang, including Tianzhu white yak and Gannan yak , Jiulong Yak, Qinghai Plateau Yak and other 12 local breeds and 1 Datong Yak breed. After long-term natural selection and artificial selection, yaks have formed a special adaptation mechanism to the high-cold and low-oxygen environment. They are the main body of the production of special livestock products in high-cold pastoral areas,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 胡江谢建鹏刘秀石斌刚王继卿
Owner GANSU AGRI UNIV
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