Method and product for wrapping single cell based on hydrogel and application in preparing universal red blood cells
A hydrogel and red blood cell technology, applied in the direction of immobilized on/in organic carriers, animal cells, vertebrate cells, etc., can solve the problems of low processing efficiency, complex preparation of biological enzymes, high cost, etc., and achieve simplified blood transfusion Programs, the effect of solving the waste of blood resources
Active Publication Date: 2018-03-06
ZHEJIANG UNIV
View PDF8 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0012] There are some limitations in the above strategies: as method (1), the preparation of biological enzymes is complicated, and the processing efficiency is low, and more importantly, it is not suitable for the D-type antigen that is embedded in the cell membrane; The retention time in the body is significantly shortened, and in vitro serological experiments show that this method cannot completely eliminate the coagulation reaction between different blood types; method (3) is expensive and the yield is too low, so it is not suitable for emergency blood transfusion
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0050] Example 1 Utilize horseradish peroxidase and hydrogen peroxide to catalyze the preparation of universal red blood cells
[0051] (1) Oleic acid (MW=282.46, 1g, 3.54mmol, 1eq) and TSTU (MW=300, 1.27g, 4.25mmol, 1.2eq) were dissolved in anhydrous DMF, and 0.1ml of triethylamine was added, under nitrogen protection, Under the condition of 35°C, react for 8 hours; stop the reaction, distill out DMF under reduced pressure, add ether to wash, and obtain oleic acid-succinimide ester (oleic acid, SE);
[0052] A DMF solution containing oleic acid-succinimide ester (MW=379.5, 189.75mg, 0.5mmol, 1eq) was slowly dropped into polyoxyethylene diamine (MW=2000, 1g, 0.5mmol, 1eq) in DMF solution (containing 0.1ml triethylamine), after completion, react for 2h, stop the reaction, distill off DMF under reduced pressure, wash with ether to obtain (E)-N-(2-(2-aminoethoxy ) ethyl-9-stearamide);
[0053] (E)-N-(2-(2-aminoethoxy) ethyl-9-stearamide) (MW=2264, 0.9g, 0.397mmol, 1eq) and succ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method and a product for wrapping a single cell based on hydrogel and an application in preparing universal red blood cells. The method comprises the following steps of: (1)synthesizing a compound I; (2) taking the compound I and horseradish peroxidase as raw materials, and carrying out an amidation reaction to obtain a compound II; (3) taking a cell suspension, adding the compound II into the cell suspension for reaction, and carrying out separation to obtain a cell system with the compound II anchored on the surface after the reaction is completed; and (4) adding atyramine hydrochloride-polysialic acid solution and a hydrogen peroxide solution into the cell system, performing an amidation reaction, and carrying out separation to obtain a single cell with the surface coated with hydrogel. The method provided by the invention can effectively wrap the hydrogel on the periphery of a single cell to realize the wrapping of the single cell, thereby constructing ahydrogel-wrapped red blood cell system for preparing universal red blood cells.
Description
technical field [0001] The invention relates to the technical field of cell interface engineering, in particular to a method and product for encapsulating single cells based on hydrogel and its application in preparing general-purpose red blood cells. Background technique [0002] Cell interface engineering is an engineering technology that uses various characteristics of the cell surface to specifically modify, control, functionalize or change the surface characteristics of biological cells by biological, chemical or physical methods, thereby transforming the corresponding behavior and function of cells. [0003] Using the method of cell interface engineering, various polymers or nanomaterials can be reacted and packaged on the surface of various cells. At present, the organisms that have been successfully encapsulated include viruses, bacteria, fungi, lower plant cells, animal cells, and even multicellular organisms. After being packaged by surface assembly of materials, ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12N11/08C12N5/078
CPCC12N5/0641C12N11/08
Inventor 王本赵玥绮范明杰唐睿康
Owner ZHEJIANG UNIV