Submicron/nano carbon fiber electrodes with high spatial resolution

Abstract

The invention relates to a submicron / nanomete carbon fiber electrode with a high spatial resolution ratio. The submicron / nanomete carbon fiber electrode with the high spatial resolution ratio is suitable for the directional collection of localized electrochemical signals of secretory cells or nerve synapses small in size. By combining two carbon fiber tip modification methods of chemical corrosionand high-temperature polishing, a carbon fiber tip of the submicron or nanometer carbon fiber electrode required for experiments is quickly, efficiently and controllably prepared. With silicone rubber as an insulating material, the modified carbon fiber tip is subjected to insulation treatment, the head section of the carbon fiber tip is partially melt by means of a polishing method to expose a probe area of the carbon fiber electrode, the high signal-to-noise ratio is ensured, and meanwhile, the dependence on equipment and methods in the prior art is avoided. A preparation method is simple and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of electrochemistry. Specifically, it relates to a submicron / nanometer carbon fiber electrode, which is suitable for collecting localized electrochemical signals directed to tiny secretory cells or nerve synapses. Background technique [0002] Electrochemical technology using carbon fiber electrodes as a detection tool is a common method for studying life activities such as cell secretion and neurosynaptic transmitter release. By applying a certain amplitude voltage to carbon fiber electrodes, specific types of released substances can be induced to occur on the surface of carbon fibers. The oxidation-reduction reaction generates an electronic signal, and the data can be analyzed and processed after being transmitted to the recording system through the carbon fiber electrode. With the popularization and application of this technology, many small secretory cells or synapses have become the research objects of...

AI Technical Summary

Problems solved by technology

However, these methods still have some shortcomings: although the laser etching method can accurately etch the carbon fiber tip, it is expensive and cannot be promoted in the short term, and it cannot be mass-produced carbon fiber electrodes

The former requires the carbon fiber to be kept at a shorter length (about 2 mm) due to factors such as surface tension and viscosity of the coating, otherwise the thickness and shape of the electroplated insulating layer at the tip of the carbon fiber cannot be controlled; The size is short and thick, and it is difficult to operate in a space-constrained environment

The advantage of the latter is that the longer carbon fiber part can be retained, but it needs special equipment to heat and melt the sleeve material at high temperature, and wire drawing and forming to complete the insulation within a limited time, which requires high operating skills and proficiency of technicians

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