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A kind of extraction method of loofah saponin

A technology of loofah saponin and extraction method, which is applied in the field of loofah saponin extraction, can solve the problems of easy destruction of saponin molecular structure, low saponin extraction rate, and low yield, and achieve the effects of avoiding damage, large output, and low cost

Active Publication Date: 2021-06-15
JINZHAI LIMIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, many techniques for extracting luffa saponins have been developed, but these techniques mostly use solvents to heat and extract luffa, and concentrate the solution to obtain saponins. Not only the yield is low, but the energy consumption is high, and the molecular structure of saponins is easily destroyed during the heating process. The extraction rate of saponin is low, which cannot meet the demand of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting luffa saponin, comprising the following steps:

[0024] (1) Soak 300kg loofah in salt water for 1min, then wash it with water. The concentration of salt water is 1.5% by weight, and the dosage is twice the weight of the loofah. After cleaning, cut it into small pieces, put it into a 1000L fermentation tank, and add 600kg of water , adding Bifidobacterium longum for fermentation, the fermentation temperature was controlled at 41°C, and sealed for 72 hours; the Bifidobacterium longum was cultured in MRS medium for 18-24 hours, and the total number of colonies reached 10 8 cfu / ml or more, to obtain a fermented seed liquid, the amount of fermented seed liquid is 3kg;

[0025] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and take the supernatant for later use;

[0026] (3) Filtering and sterilizing the clear liquid obtained in step (2) through a microporous membrane with a pore size of 1 μm;

[0027] (4) Use the HPD101 mac...

Embodiment 2

[0029] A method for extracting luffa saponin, comprising the following steps:

[0030] (1) Soak 500kg of loofah in salt water for 5 minutes, and then wash it with water. The concentration of salt water is 0.5% by weight, and the dosage is 5 times the weight of the loofah. After cleaning, cut it into small pieces, put it into a 1000L fermentation tank, and add 500kg of water , adding Bifidobacterium breve to ferment, the fermentation temperature is controlled at 36°C, sealed and fermented for 24 hours; Bifidobacterium breve is cultured in MRS medium for 18~24h, and the total number of colonies reaches 10 8 If the cfu / ml is above, the fermented seed liquid is obtained, and the consumption of the fermented seed liquid is 25kg;

[0031] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and take the supernatant for later use;

[0032] (3) Sterilize the clear liquid obtained in step (2) by filtering through a microporous membrane with a pore size of 0.5 μm;

...

Embodiment 3

[0035] A method for extracting luffa saponin, comprising the following steps:

[0036] (1) Soak 500kg of loofah in salt water for 3 minutes, and then wash it with water. The concentration of salt water is 1.0% by weight, and the dosage is 3 times the weight of the loofah. After cleaning, cut it into small pieces, put it into a 1000L fermentation tank, and add 500kg of water , adding Bifidobacterium infantis to ferment, the fermentation temperature is controlled at 37°C, sealed and fermented for 48 hours; Bifidobacterium infantis is cultured in MRS medium for 18~24 hours, and the total number of colonies reaches 10 8 cfu / ml or more, to obtain a fermented seed liquid, the amount of fermented seed liquid is 15kg;

[0037] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and take the supernatant for later use;

[0038] (3) Sterilize the clear liquid obtained in step (2) by filtering through a microporous membrane with a pore size of 0.5 μm;

[0039] (4) Us...

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PUM

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Abstract

The invention discloses a method for extracting loofah saponins, which comprises the following steps: washing the loofah and cutting it into small pieces, adding water 1 to 2 times the weight of the loofah, and adding bifidobacteria for fermentation; centrifuging the fermented liquid, and taking the clear liquid Standby; the clear liquid is sterilized by filtration through a microporous membrane; the filtrate is selectively separated by continuous chromatography to obtain luffa saponin. The invention has the advantages of: adopting the fermentation method to extract, eliminating the heating process in the chemical extraction, avoiding the destruction of luffa saponin due to heating, and making the extraction rate higher; reducing the use of chemical agents and improving the quality of luffa saponin; adopting large-scale production , large output and low cost.

Description

technical field [0001] The invention belongs to the field of fermentation industry, and in particular relates to a method for extracting luffa saponin. Background technique [0002] Loofah is also called Tianloofah, pretty melon, hanging melon, water melon, etc. It originated in India and was introduced into my country in the Tang Dynasty. Loofah is widely cultivated throughout the north and south of my country, and is one of the main vegetables in summer and autumn, and the roots, vines, leaves, seeds and loofah collaterals of loofah can be used as medicine. Modern science and technology analysis of loofah contains protein, fat, carbohydrates, calcium, phosphorus, iron and vitamin B1, vitamin C, plant mucus, xylose gum, loofah bitterness, citrulline and other substances. Recent studies have shown that loofah contains Also rich in saponins. Therefore, many techniques for extracting luffa saponins have been developed, but these techniques mostly use solvents to heat and ext...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04B01D61/14B01D15/08B01D15/42B01D9/02C12R1/01
CPCB01D9/00B01D15/08B01D15/424B01D61/147C12P1/04C12N1/205C12R2001/01
Inventor 刘新利陈蕾卢涛吴善良
Owner JINZHAI LIMIN BIOTECH CO LTD