Anti-CD19 antibody and preparation method and use thereof

A CDR-H1, CDR-L1 technology, applied in the field of anti-CD19 antibody and its preparation, can solve the problems of poor persistence of CART cells, decreased affinity, and inability of CART cells to clear plasma cells
CN107793480AActive Publication Date: 2018-03-13SHANGHAI GENBASE BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI GENBASE BIOTECH CO LTD
Publication Date
2018-03-13

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Abstract

The invention relates to the technical field of biotechnology and particularly relates to an anti-CD19 antibody and a preparation method and use thereof. The anti-CD19 antibody comprises a heavy chainvariable region and a light chain variable region. The complementarity determining region of the heavy chain variable region comprises CDR-H1 with an amino acid sequence shown in the formula of SEQ ID No. 1, CDR-H2 with an amino acid sequence shown in the formula of SEQ ID No. 2 or 3 and CDR-H3 with an amino acid sequence shown in the formula of SEQ ID No. 4. The complementarity determining region of the light chain variable region comprises CDR-L1 with an amino acid sequence shown in the formula of SEQ ID No. 5, CDR-L2 with an amino acid sequence shown in the formula of SEQ ID No. 6 and CDR-L3 with an amino acid sequence shown in the formula of SEQ ID No. 7. The FMC63 scFv is screened in affinity maturation through a phage display technology so that a high affinity single chain antibodyagainst CD19 is obtained.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an anti-CD19 antibody and its preparation method and application. Background technique

[0002] CD19 antigen (B lymphocyte antigen CD19, CD19) is a protein specifically expressed on the surface of human B cells. CD19 is widely expressed on the surface of B cells at various developmental stages and plays an important role: CD19 acts as a B cell receptor (B cell receptor, BCR) co-receptor (co-receptor), which can reduce antigen-mediated B cell receptor Signaling threshold required for antigen receptor-dependent stimulation; B-cell receptor activation is dependent on phosphorylation of the intracellular domain of CD19 followed by recruitment of Src kinase and PI3K kinase to fully activate B cells. Fully mature B cells are called plasma cells, and plasma cells will lose the expression of CD19 antigen after fully mature.

[0003] Hematological tumors associated with malignant proliferat...

Claims

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