Freeze drying protective agent of lactic acid bacteria
A technology for drying protective agent and lactic acid bacteria, which is applied in the preservation of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of general protection effect and inability to protect lactic acid bacteria, etc., and achieves the effect of improving the protective effect and improving the survival rate.
Inactive Publication Date: 2018-03-20
合肥民祯健康科技有限公司
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- Abstract
- Description
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- Application Information
AI Technical Summary
Problems solved by technology
[0003] At present, most of the freeze-drying protective agents used for lactic acid bacteria in the market are single-substance freeze-drying protective agents, which cannot fully protect lactic acid bacteria, and the protective effect is average.
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] The invention discloses a lactic acid bacteria freeze-drying protective agent, which consists of the following components according to mass percentage: 8% skimmed milk powder, 1% trehalose, 2% glycerin, 0.5% PVP, 1.5% sodium glutamate, and the balance for water.
Embodiment 2
[0019] A lactic acid bacteria freeze-drying protective agent is composed of the following components according to mass percentage: 12% skimmed milk powder, 1.5% trehalose, 3% glycerin, 1% PVP, 1% sodium glutamate, and the balance is water.
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The invention discloses a freeze drying protective agent of lactic acid bacteria. The freeze drying protective agent is characterized in that the freeze drying protective agent is prepared from the following ingredients in parts by weight: 8-12% of dried skim milk, 1-2% of mycose, 2-4% of glycerinum, 0.5-1% of PVP (Polyvinyl Pyrrolidone), 1-3% of sodium glutamate and the balance of water. Different types of protective agent are mixed to form compound protective agent, the protective agents perform respective functions in a freeze drying process and also mutually have a synergistic effect, so that a protection function for the lactic acid bacteria is improved, and a survival rate after the lactic acid bacteria are subjected to freeze drying is improved.
Description
technical field [0001] The invention relates to a lactic acid bacteria freeze-drying protective agent, which is applied in the technical field of lactic acid bacteria processing. Background technique [0002] With the development of society in recent years, lactic acid bacteria products have gradually attracted people's attention. In the process of lactic acid bacteria processing, lactic acid bacteria need to be freeze-dried, that is, the microbial cells to be preserved are sublimated in a vacuum and frozen state, and finally dried and frozen. Drying is a multi-step process, which will produce a variety of stresses to deform lactic acid bacteria, such as low temperature stress, freezing stress, etc., which often cause changes in cell membrane permeability of lactic acid bacteria, protein denaturation and inactivation, and PH dynamic balance is destroyed. In order to protect the activity of the product, a protective agent needs to be added during the freeze-drying process. ...
Claims
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IPC IPC(8): C12N1/04C12R1/645
CPCC12N1/04
Inventor 宋建民王德海宛荣生
Owner 合肥民祯健康科技有限公司