Buffer solution used for nucleic acid purification, nucleic acid purification kit and application thereof

Abstract

The invention discloses a buffer solution used for nucleic acid purification, a nucleic acid purification kit and application thereof. The buffer solution used for nucleic acid purification comprisesTris-HCl, NaCl, NaN3 and polyethylene glycol, wherein the final concentration of Tris-HCl is 100mmol / L-1mol / L, the final concentration of NaCl is 1mol / L-5mol / L, the final concentration of NaN3 is 0.02%w / v-1.00%w / v, and the final concentration of polyethylene glycol is 1%w / v-30%w / v. According to the buffer solution for nucleic acid purification disclosed by the invention, several reagents are subjected to component and proportioning optimization, so that the buffer solution can be well applied to purification of nucleic acid through magnetic beads, and quality of nucleic acid purified by the magnetic beads can be improved. The kit of the buffer solution disclosed by the invention can completely replace expensive import kits, and the research cost of nucleic acid purification can be greatlyreduced simultaneously when the nucleic acid purification quality can be guaranteed.

Description

technical field [0001] The present application relates to the field of nucleic acid purification reagents, in particular to a buffer for nucleic acid purification, a nucleic acid purification kit and applications thereof. Background technique [0002] High-purity and high-concentration nucleic acid samples have always been one of the important conditions for obtaining good experimental results in molecular biology experiments. Although there are many methods for preparing single-stranded DNA, double-stranded DNA and RNA; but there are not many fast, low-cost and high-throughput methods that are suitable for all types of nucleic acids, and the magnetic bead purification method is one of them one. [0003] Magnetic bead purification of nucleic acid is a new nucleic acid purification method developed in recent years. Compared with the traditional purification technology in the past, magnetic bead purification does not require centrifugation or filtration, and can be done manua...

AI Technical Summary

Problems solved by technology

Among them, Beckman is the most famous, and its Ampure XP magnetic bead kit has become a star product in the industry, but its high price has a great impact on the production cost of the entire sequencing industry, which to a certain extent restricts the promotion and wide application of gene sequencing technology in my country

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