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A method for blood cell collection and ultrastructural observation of sea mussels

An ultrastructure and blood cell technology, applied in animal cells, invertebrate cells, scientific instruments, etc., can solve the problems of different activities, shapes, changes, and inappropriateness of blood cells, and achieve simple, repeatable, and easy-to-operate blood cell separation methods Effect

Active Publication Date: 2020-05-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0004] As we all know, improper handling of blood cells after isolation will lead to changes in activity and morphology of blood cells that are different from those in the body; and improper selection of blood collection sites and improper blood collection operations may contain tissue fluid or other body fluids in the blood taken. The analysis results will have a great impact, which may be one of the reasons why there is no unified standard and conclusion for the current shellfish blood cell classification

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  • A method for blood cell collection and ultrastructural observation of sea mussels
  • A method for blood cell collection and ultrastructural observation of sea mussels
  • A method for blood cell collection and ultrastructural observation of sea mussels

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Embodiment

[0019] Scapharca broughttonii is a typical marine clam shellfish, which has the characteristics of delicious taste, rich nutrition, strong adaptability, fast growth, and remarkable ecological environment restoration effect. One of the advantages of aquatic products. Studies have shown that cockles have strong clearance and defense capabilities against common pathogens, high genetic diversity, rich genetic variation, strong resistance to stress, obvious breeding indicators, and easy to expand their distribution range. At the same time as the research on the genetics and breeding of cockles, people began to pay attention to the analysis of blood immune function of this species. Through collection, fixation, separation and electron microscope observation of the blood cells of the cockles, it is known that the blood cells of the cockles are clearly classified, all kinds of blood cells have immune responses to the infection of pathogenic bacteria, and the division of immune functio...

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Abstract

The invention provides a method for hemocyte collection and ultra microstructure observation of seawater arcidae shellfish and belongs to the technical field of marine organism. The method can be usedfor collecting blood of arcidae shellfish living bodies, and the blood collecting site is blood sinus in adductor muscle close to the edge of the shell. The method disclosed by the invention can be used for collecting and separating blood cells in the condition of not damaging the shell body by improvement on the existing method for hemocyte collection and ultra microstructure observation of seawater arcidae shellfish, and can perform ultra microstructure observation on the collected blood cells. The method can overcome the limitations of less quantity and small figure of experiment shells, is simple and convenient in operation, and preserves the activated state of the blood cell and the normal form of the blood cell in an organism, and shell bodies can still survive after blood collection and restore to the normal healthy state, thus meeting the requirements of repeated experiment and comparison experiment.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for collecting blood cells and observing the ultrastructure of shellfish of the sea cockle family. Background technique [0002] A large number of studies believe that shellfish do not have a specific immune system and cannot produce antibodies to resist the infection of foreign substances. However, shellfish, as a relatively primitive invertebrate, also have a set of effective immune systems in the long-term evolution process. The defense method is used to protect the body, and the phagocytosis, encapsulation and production of active oxygen molecules of blood cells play such a role (Li Taiwu et al., Blood cells of variegated abalone, Acta Aquatica Sinica, 2007, 31(Suppl): 12-17) . As the formed component of blood, blood cells are not only the components of the body, but also the main immune cells of the body. The data of hematological parameters ca...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N23/2208G01N23/2202C12N5/07
CPCC12N5/0601G01N23/2202G01N23/2208G01N2223/072G01N2223/612
Inventor 周丽青杨爱国吴彪孙秀俊刘志鸿赵庆张高伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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