Focusing controllable super-resolution microscopic device based on spherical micro nano liquid drop lens

Abstract

The invention discloses a focusing controllable super-resolution microscopic device based on a spherical micro nano liquid drop lens; the focusing controllable super-resolution microscopic device comprises a laser, a reflector, a first lens group, a dichroscope, a linear polarizer, a microscopic object lens, a packaging box, a spherical micro nano liquid drop lens, a micro flow tube, a micro displacement bench, a sample table, a second lens group, an imaging device, and a computer control system. The computer control system controls the micro flow tube to release liquid drops of certain volumeinto the packaging box or absorb liquid drops of certain volume, thus regulating the spherical micro nano liquid drop lens radius, and zooming the spherical micro nano liquid drop lens. An existing micro nano structure focusing characteristics are uncontrollable; the focusing controllable super-resolution microscopic device based on the spherical micro nano liquid drop lens can solve said problems, uses the spherical micro nano liquid drop lens to form photon nanometer injection, and regulates the spherical micro nano liquid drop lens focusing characteristics, thus scanning samples at different depths under non-mechanical structure vibration conditions; the micro displacement bench is combined to regulate the sample table, thus scanning in the horizontal direction, and finishing three dimensional super-resolution microscopic imaging of the samples.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a focus-controllable super-resolution microscopic device based on a spherical micro-nano droplet lens. Background technique [0002] Since Abbe proposed the concept of the optical diffraction limit in 1873, how to break through the optical diffraction limit and obtain higher-quality high-resolution images has been a hot topic in academic research. Until the emergence of super-resolution optical imaging technology, it broke through the resolution limit of conventional optical microscopes and provided unprecedented solutions for research in the fields of life sciences and biological cytology. At present, the mainstream super-resolution optical microscopy technology is mainly divided into: target switching and reading microscopy technology represented by fluorescence emission loss microscopy proposed by S.W.Hell et al. and photosensitive positioning microscopy technology p...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the disadvantages of uncontrollable focusing characteristics when the existing micro-nano structure realizes super-resolution microscopic imaging in the near field, and proposes a focus-controllable super-resolution microscopic device based on a spherical micro-nano droplet lens

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