A nanoparticle tumor diagnosis and treatment combined preparation and its preparation method and application

A nanoparticle and tumor diagnosis technology, which is applied in the field of tumor diagnosis and treatment preparations and biomedical materials, can solve the problems of poor targeting function, low drug loading, high immunogenicity, etc., and achieve extended half-life, good biological safety, and reduced Effects of Laser Energy

Active Publication Date: 2018-12-28
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanocarrier systems such as microcapsules, liposomes, and polymer micelles that have been studied more now generally have problems such as high immunogenicity, low drug loading, easy drug leakage, poor targeting function, and easy aggregation.

Method used

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  • A nanoparticle tumor diagnosis and treatment combined preparation and its preparation method and application
  • A nanoparticle tumor diagnosis and treatment combined preparation and its preparation method and application
  • A nanoparticle tumor diagnosis and treatment combined preparation and its preparation method and application

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Effect test

Embodiment 1

[0039] The preparation process of the nanoparticle tumor diagnosis and treatment combination preparation of the present invention (diagnosis and treatment preparation of Prussian blue nanoparticles with high-efficiency adsorption of radioactive rare earth element lutetium) is as follows: figure 1 As shown, taking Prussian blue nanoparticles, radioactive rare earth element lutetium and platelet membrane as examples, the following steps are included:

[0040] (1) Preparation of citric acid-modified Prussian blue nanoparticles by hydrothermal method: heat and stir the citric acid solution of ferrous and ferric salts in a water bath, then cool to room temperature to obtain a dark blue liquid, which is centrifuged and cleaned and placed in the freezer Freeze-dried in a dryer into freeze-dried powder. The preparation process was adjusted so that the prepared nanoparticles had a particle size of about 100 nm, uniform size and good dispersion.

[0041] (2) Adsorption of radioactive l...

Embodiment 2

[0044] The characterization data of the nanoparticle tumor diagnosis and treatment combination preparation of the present invention (diagnosis and treatment preparation of Prussian blue nanoparticles that efficiently adsorb radioactive rare earth element lutetium) are as follows:

[0045] The peak of the absorption spectrum of the citric acid-modified Prussian blue nanoparticle solution prepared by hydrothermal method is about 700 nm, which is the characteristic absorption peak of the Prussian blue nanoparticle. The hydration radius is 95.72±3.44 nm, the surface potential is -27.86±0.90 mV, and the aqueous solution of Prussian blue nanoparticles is uniform and stable without any aggregation phenomenon. The shape and size of Prussian blue nanoparticles were observed by transmission electron microscope, and it was found that its shape was regular hexahedron, and the particle size was about 100 nm, as shown in the attached figure 2 shown.

[0046] Through the determination of t...

Embodiment 3

[0048] In vivo SPECT / CT imaging, specifically including the following steps:

[0049] (1) The selected tumor size is about 150 mm 3 Tumor-bearing nude mice (the tumor model is human colon cancer cell HT-29 model).

[0050] (2) Tail vein injection concentration is 10 mg mL -1 100 μL of Prussian blue nanoparticles that efficiently adsorb the radioactive rare earth element lutetium.

[0051] (3) SPECT / CT imaging of nanoparticles monitored by SPECT / CT in 24 hours.

[0052] The experimental results are attached Figure 5 As shown, after 24 hours of drug injection, SPECT / CT imaging light signals can be detected in the tumor site of nude mice. This indicates that the nanoparticles can be massively accumulated in the tumor site of nude mice, and the tumor site can be imaged by SPECT.

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Abstract

The invention discloses a combined preparation for nanoparticle tumor diagnoses and treatment and a preparation method and an application thereof. The preparation adsorbs radioactive rare earth element lutetium ions with prussian blue nanoparticles which are good in biocompatibility and platelet membranes coat the surfaces of the nanoparticles. The prussian blue nanoparticles used in the preparation can be used for photo-acoustic imaging and generates energy through near infrared laser irradiation while monitoring a diagnoses and treatment agent in real time so as to improve the temperature tokill tumor cells for photothermal therapy. Meanwhile, the radioactive rare earth element lutetium ions also can realize SEPCT imaging and radiotherapy functions. The platelet membranes coating the surfaces of the nanoparticles can improve the recognition of tumor tissues so as to realize a function of targeting tumors. In addition, the nano diagnoses and treatment preparation is high in biosafety, good in tumor targeting property, simple in preparation method, low in cost and good in repeatability, and has wide application prospect in the field of tumor diagnoses and treatment.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to the technical field of tumor diagnosis and treatment preparations, in particular to a nanoparticle tumor diagnosis and treatment combination preparation and a preparation method thereof. Background technique [0002] In recent years, the research on tumor treatment has become a hot research topic in materials chemistry, biophysics, pharmacy, radiology, medical imaging and other disciplines, but the clinical treatment effect is still not ideal. In order to better treat tumors, it is necessary to apply the latest technical methods and design concepts in materials science, physics and biomedicine, design intelligent targeted drug carriers, and perform real-time detection through medical imaging technology imaging methods, and Combine various treatment methods to better treat tumors. [0003] Radiotherapy is a local treatment method for tumors treated with radiation. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K51/12A61K51/04A61K51/02A61K41/00A61P35/00A61K103/30
CPCA61K41/0052A61K49/221A61K49/225A61K51/02A61K51/04A61K51/1251
Inventor 王凡李小达梁晓龙史继云贾兵马晓途高瀚男张欣姚美男邵楠张旭侯睿
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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